Virology home
Publications
Japanese
Research themes
We are committed to conducting basic and applied research on mosquito-borne
flaviviral diseases, and the development of diagnostics,
treatments and preventive methods for tropical and emerging virus diseases.
The viruses under research includes,
Dengue virus
West Nile virus
JE virus
Nipas virus
SARS virus.
Papers published
2015
Hayasaka D, Shimada S, Aoki K, Takamatsu Y, Uchida L, Horio M, Fuxun Y, Morita K. Epidemiological survey of severe fever with thrombocytopenia syndrome virus in ticks in Nagasaki, Japan. Trop Med Health. Accepted
Shimada S, Posadas-Herrera G, Aoki K, Morita K, Hayasaka D. Therapeutic effect of post-exposure treatment with antiserum on severe fever with thrombocytopenia syndrome (SFTS) in a mouse model of SFTS virus infection. Virology. 2015 Mar 25;482:19-27.
Takamatsu Y, Morita K, Hayasaka D. A unique amino acid substitution in NS2A protein of Japanese encephalitis virus affects virus propagation in vitro but not in vivo. J Virol. 2015 Mar 18.
Nagata N, Iwata-Yoshikawa N, Hayasaka D, Sato Y, Kojima A, Kariwa H, Takashima I, Takasaki T, Kurane I, Sata T, Hasegawa H. The pathogenesis of 3 neurotropic flaviviruses in a mouse model depends on the route of neuroinvasion after viremia. J Neuropathol Exp Neurol. 2015 Mar;74(3):250-60.
Yuki Takamatsu, Takeshi Nabeshima, Thuy Thi Thu Nguyen, Dinh Thi Dang, Ly Hoai Linh Pham, Hang Thi Pham, Lien Thi Nam Nguyen, Huong Ly Minh Phu, Thuy Thu Bui, Mai Thi Quynh Le, Kouichi Morita, Futoshi Hasebe. A Dengue virus serotype 4-dominated outbreak in central Vietnam, 2013. Journal of Clinical Virology. Vol.66, 24-26, 2015
Kyaw Zin Thant, Mya Myat Ngwe Tun, Maria del Carmen Parquet, Shingo Inoue, Yee Yee Lwin, Sanda Lin, Kay Thi Aye, Pe Thet Khin, Tin Myint, Khin Htwe, Takeshi Nabeshima and Kouichi Morita. Molecular Epidemiology of Dengue Viruses Co-circulating in Upper Myanmar in 2006. Tropical Medicine and Health Vol. 43, 21-27, 2015
Shirai K, Hayasaka D, Kitaura K, Takasaki T, Morita K, Suzuki R, Kurane I. Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus. Arch Virol. Vol.160(3):765-775, 2015
2014
Watanabe Y, Ito T, Ibrahim MS, Arai Y, Hotta K, Phuong HV, Hang NL, Mai LQ, Soda K, Yamaoka M, Poetranto ED, Wulandari L, Hiramatsu H, Daidoji T, Kubota-Koketsu R, Sriwilaijaroen N, Nakaya T, Okuno Y, Takahashi T, Suzuki T, Ito T, Hotta H, Yamashiro T, Hayashi T, Morita K, Ikuta K, Suzuki Y. A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity. Biosens Bioelectron. Vol.65C:211-219. 2014
Leo Uchida, Lyre Anni Espada-Murao, Yuki Takamatsu, Kenta Okamoto, Daisuke Hayasaka, Fuxun Yu, Takeshi Nabeshima, Corazon C. Buerano, and Kouichi Morita. The dengue virus conceals double-stranded RNA in the intracellular membrane to escape from an interferon response. Scientific Reports. 4:7395. doi: 10.1038/srep07395. 2014
Bijaya Gaire, Komal Raj Rijal, Biswas Neupane, Pravin Paudyal, Ishan Gautam, Megha Raj Banjara, Kouichi Morita and Basu Dev Pandey. Prevalence of dengue vector in relation to dengue virus infection in central region of Nepal. Dengue Bulletin.Vol.38:96-107, 2014
C. Murch, J.Lindenmayer, K. Pandey, G. Kaufman, D. D. Joshi, K. Morita , B. D.Pandey, EVALUATING THE USE OF EQUINE RABIES IMMUNOGLOBULIN (EQUIRAB) FOR POST EXPOSURE PROPHYLAXIS IN KATHMANDU, NEPAL, Nepalese Vet. J. 31:88-95, 2014
Takeshi Nabeshima, Shingo Inoue, Kenta Okamoto, Guillermo Posadas-Herrera, Fuxun Yu, Leo Uchida, Akitoyo Ichinose, Miako Sakaguchi, Toshihiko Sunahara, Corazon C. Buerano, Florencio P. Tadena, Ildefonso B. Orbita, Filipinas F. Natividad, Kouichi Morita. Tanay virus, a new species of virus isolated from mosquitoes in the Philippines. Journal of General Virology, Vol.95:1390-5. 2014
Aoki K, Shimada S, Simantini DS, Ngwe Tun MM, Buerano CC, Morita K, Hayasaka D. Type-I interferon response affects an inoculation dose-independent mortality in mice following Japanese encephalitis virus infection. Virol J. 11; 105, 2014
Ngwe Tun MM, Aoki K, Senba M, Buerano CC, Shirai K, Suzuki R, Morita K, Hayasaka D. Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus. Sci Rep. 4:5344, 2014
Ngwe Tun MM, Thant KZ, Inoue S, Nabeshima T, Aoki K, Kyaw AK, Myint T, Tar T, Maung KTT, Hayasaka D, Morita K. Emergence of East Central South African Genotype of Chikungunya Virus in Myanmar, 2010. Emerg Infect Dis. 20:1378-1381, 2014
Tomoko Abe, Ayumi Sando, Fumiteru Teraoka, Tadamune Otsubo, Kouichi Morita, Hiroaki Tokiwa , Kiyoshi Ikeda , Takashi Suzuki , Kazuya I.P.J. Hidari. Computational design of a sulfoglucuronide derivative fitting into a hydrophobic pocket of dengue virus E protein. Biochemical and Biophysical Research Communications. Vol449:32-37, 2014
Le Xuan Luat, Mya Myat Ngwe Tun, Corazon C. Buerano, Kotaro Aoki, Kouichi Morita and Daisuke Hayasaka. Pathologic potential of variant clones of the Oshima strain of Far Eastern subtype tick-borne encephalitis virus. Tropical Medicine and Health. Vol. 42:15-23, 2014
Fumihiko Yasui, Michinori Kohara , Masahiro Kitabatake , Tetsu Nishiwaki , Hideki Fujii, Chise Tateno, Misako Yoneda, Kouichi Morita, Kouji Matsushima , Shigeo Koyasu, Chieko Kai. Phagocytic cells contribute to the antibody-mediated elimination of pulmonary-infected SARS coronavirus. Virology 454-455: 157-168. 2014
Muhareva Raekiansyah, Lyre Anni Espada-Murao, Kenta Okamoto, Toru Kubo, Kouichi Morita . Dengue virus neither directly mediates hyperpermeability nor enhances TNF-α-induced permeability in vitro. Japanese Journal of Infectious Diseases, Vol.67:86-94, 2014
Yuki Takamatsu, Kenta Okamoto, Duc Tuan Dinh, Fuxun Yu, Daisuke Hayasaka, Leo Uchida, Takeshi Nabeshima, Corazon C. Buerano and Kouichi Morita, NS1’ protein expression facilitates production of Japanese encephalitis virus in avian cells and embryonated chicken eggs, Journal of General Virology, 95, 373-383. 2014
2013
Biswas Neupane, Komal Raj Rijal, Gyan Bahadur Aryal,Yogendra Shah, Megha Raj Banjara, Jeevan Bahadur Sherchand, Kouichi Morita and Basu Dev Pandey. Clinical and laboratory features of dengue fever in the southern lowlands of Nepal, Dengue Bulletin, Vol.37:1-10, 2013
Haorile Chagan-Yasutan, Lishomwa C. Ndhlovu, Talitha Lea Lacuesta, Toru Kubo, Prisca Susan A. Leano, Toshiro Niki, Shigeru Oguma, Kouichi Morita, Glen. M. Chew, Jason D. Barbour, Elizabeth Freda O. Telan, Mitsuomi Hirashima, Toshio Hattori, Efren M. Dimaano. Galectin-9 Plasma Levels Reflect Adverse Hamatological and Immunological Features in Acute Dengue Virus Infection J.Clin.Virol. Vol.58. 635-640, 2013
Khalid A Enan, Takeshi Nabeshima, Toru Kubo, Corazon C Buerano, Abdel Rahim M . El Hussein, Isam M Elkhidir, Eltahir AG Khalil and Kouichi Morita. Survey of causative agents for acute respiratory infections among patients in Khartoum- State, Sudan, 2010- 2011. Virology Journal, 10:312. 2013
Yuki Takamatsu, Leo Uchida, Phan Thi Nga, Kenta Okamoto, Takeshi Nabeshima, Dang Thi Thu Thao, Do Thien Hai, Nguyen Thi Tuyet, Hoang Minh Duc, Le Xuan Luat, Futoshi Hasebe and Kouichi Morita. An approach for differentiating Echovirus 30 and Japanese encephalitis virus infections in acute meningitis/encephalitis: a retrospective study of 103 cases in Vietnam. Virology Journal 10:280, 2013
Basu D. Pandey, Takeshi Nabeshima, Kishor Pandey, Saroj P. Rajendra, Yogendra Shah, Bal R. Adhikari, Govinda Gupta, Ishan Gautam, Mya M. N. Tun, Reo Uchida, Ichiro Kurane and Kouichi Morita. First Isolation of Dengue Virus from the 2010 Epidemic in Nepal. Tropical Medicine and Health Vol. 41 No. 3: 1-9, 2013
Nguyen Thanh Thuy, Tran Quang Huy, Phan Thi Nga, Kouichi Morita, Irene Dunia, Lucio Benedetti. A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line. Virology Vol.444, 337-342, 2013
Chris Lauber, Jelle J. Goeman, Maria del Carmen Parquet, Phan Thi Nga4, Eric J. Snijder1, Kouichi Morita, Alexander E. Gorbalenya, The Footprint of Genome Architecture in the Largest Genome Expansion in RNA Viruses. PLoS Pathogens 9 (7): e1003500., July 2013
Hayasaka D, Shirai K, Aoki K, Nagata N, Simantini DS, Kitaura K, Takamatsu Y, Gould E, Suzuki R, Morita K. TNF-α acts as an immunoregulator in the mouse brain by reducing the incidence of severe disease following Japanese encephalitis virus infection. PLoS One. August 5;8(8):e71643. 2013
Hayasaka D, Aoki K, Morita K. Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop -mediated isothermal amplification. Virology Journal Mar 4;10:68. 2013
Ngwe Tun MM, Thant KZ, Inoue S, Kurosawa Y, Lwin YY, Lin S, Aye KT, Thet Khin P, Myint T, Htwe K, Mapua CA, Natividad FF, Hirayama K, Morita K. Serological characterization of dengue virus infections observed among dengue hemorrhagic fever/dengue shock syndrome cases in Upper Myanmar. J.Med.Virol. Vol.85:1258-1266, 2013
Kwallah AO, Inoue S, Muigai AW, Kubo T, Sang R, Morita K, Mwau M., A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus. J Virol Methods. (May 18) ;Vol.193(1):23-27. 2013
2012
Hidari KI, Ikeda K, Watanabe I, Abe T, Sando A, Itoh Y, Tokiwa H, Morita K, Suzuki. T 3-O-sulfated glucuronide derivative as a potential anti-dengue virus agent. Biochem Biophys Res Commun. 2012 Aug 3;424(3):573-8.
Lauber C, Ziebuhr J, Junglen S, Drosten C, Zirkel F, Nga PT, Morita K, Snijder EJ, Gorbalenya AE. Mesoniviridae: a proposed new family in the order Nidovirales formed by a single species of mosquito-borne viruses. Arch Virol. Vol.157, 1623-1628. 2012
Kenta Okamoto, Hitomi Kinoshita, Maria del Carmen Parquet, Muhareva Raekiansyah, Daisuke Kimura, Katsuyuki Yui, Mohammed Alimul Islam, Futoshi Hasebe, Kouichi Morita. Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. Journal of General Virology. Vol. 93(4): 761-770. 2012
Futoshi Hasebe, Nguyen Thi Thu Thuy, Shingo Inoue, Fuxun Yu, Yoshihiro Kaku, Shumpei Watanabe, Hiroomi Akashi, Dang Tuan Dat, Le Thi Quynh Mai, Kouichi Morita. Serologic Evidence of Nipah Virus Infection in Bats, Vietnam. Emerging Infectious Diseases. Vol.18(3):536-7. 2012
Takahisa Furuta, Lyre Anni Murao, Nguyen Thi Phuong Lan, Nguyen Tien Huy, Vu Thi Que Huong, Tran Thi Thuy, Vo Dinh Tham, Cao Thi Phi Nga, Tran Thi Ngoc Ha, Yasukazu Ohmoto, Mihoko Kikuchi, Kouichi Morita, MichioYasunami, Kenji Hirayama, and Naohiro Watanabe, Association of Mast Cell-Derived VEGF and Proteases in Dengue Shock Syndrome. PLoSNTD, Vol.6:e1505.2012
2011
Tran Thi Ngoc Ha, Nguyen Tien Huy, Lyre Anni Murao, Nguyen Thi Phuong Lan, Tran Thi Thuy, Ha Manh Tuan, Cao Thi Phi Nga, Vo Van Tuong, Tran Van Dat, Mihoko Kikuchi, Michio Yasunami, Kouichi Morita, Vu Thi Que Huong, Kenji Hirayama. Elevated Levels of Cell-Free Circulating DNA in Patients with Acute Dengue Virus Infection. Plos One 6(10):e25969. 2011
Phan Thi Nga, Maria del Carmen Parquet, Chris Lauber, Manmohan Parida, Takeshi Nabeshima, Fuxun Yu, Nguyen Thanh Thuy, Shingo Inoue, Takashi Ito, Kenta Okamoto, Akitoyo Ichinose, Eric J. Snijder, Kouichi Morita, Alexander E. Gorbalenya. Discovery of the First Insect Nidovirus, a Missing Evolutionary Link in the Emergence of the Largest RNA Virus Genomes. PLoS Pathogens 7 (9): e1002215. 2011
Lyre Anni Espada-Murao and Kouichi Morita. Delayed cytosolic exposure of Japanese encephalitis virus double-stranded RNA impedes interferon activation and enhances viral dissemination in porcine cells. Journal of Virology,Vol.85(13):6736-6749, 2011
Duc Tuan Dinh, Mai Thi Quynh Le, Cuong Duc Vuong, Futoshi Hasebe and Kouichi Morita. An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses. Tropical Medicine and Health,Vol.39(1):3-7, 2011
Wichit S, Jittmittraphap A, Hidari KI, Thaisomboonsuk B, Petmitr S, Ubol S, Aoki C, Itonori S, Morita K, Suzuki T, Suzuki Y, Jampangern W. Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito calls. Microbiol Immunol. Vol. 55(2) : 135-140,2011
2010
Duc Tuan Dinh, Mai Thi Quynh Le, Cuong Duc Vuong, Futoshi Hasebe and Kouichi Morita. An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses. Tropical Medicine and Health,Vol.39(1):3-7, 2011
Wichit S, Jittmittraphap A, Hidari KI, Thaisomboonsuk B, Petmitr S, Ubol S, Aoki C, Itonori S, Morita K, Suzuki T, Suzuki Y, Jampangern W. Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito calls. Microbiol Immunol. Vol. 55(2) : 135-140,2011
Yasui F., Kai C., Saito K., Inoue S., Yoneda M., Morita K., Mizuno
K., Kohara M. Analysis of the mechanism by which BALB/c mice having
prior immunization with nucleocapsid protein of SARS-CoV develop severe
pneumonia after SARS-CoV
infection. Procedia in Vaccinology. Vol.2:42-48, 2010
Michael O Baclig, Leonora TS Gervacio, Lady-Anne C Suarez, Corazon C Buerano, Ronald R Matias, Atsushi Kumatori, Shingo Inoue, Kouichi Morita, Filipinas F Natividad and Futoshi Hasebe. Flow Cytometric Analysis of Dengue Virus-Infected Cells in Peripheral Blood. Southeast Asian J Trop Med Public Health, Vol.41(6): 1352-1358, 2010
Guillermo Posadas-Herrera, Shingo Inoue, Isao Fuke, Yuko Muraki, Cynthia A. Mapua, Afjal Hossain Khan, Maria del Carmen Parquet, Sadao Manabe, Osamu Tanishita, Toyokazu Ishikawa, Filipinas F. Natividad, Yoshinobu Okuno, Futoshi Hasebe, Kouichi Morita. Development and evaluation of a formalin-inactivated West Nile Virus vaccine (WN-VAX) for a human vaccine candidate. Vaccine 28, 7939-7946, 2010
Daisuke Katoa, Shota Era, Ippei Watanabe, Masataka Arihara, Nobuo Sugiura, Koji Kimata, Yasuo Suzuki, Kouichi Morita, Kazuya I.P.J. Hidari, Takashi Suzuki. Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein. Antiviral Research, Vol.88:236-243< 2010
Kouichi Morita. West Nile fever: Expected vaccine for the future, Clinical Inspection, Vol.54:1411-1446, 2010
Kenta Okamoto, Yushirou Endo, Shingo Inoue, Takeshi Nabeshima,
Phan Thi Nga, Posadas H. Guillermo, Fuxun Yu, Do Phuong Loan, Bui Minh
Trang, Filipinas F. Natividad, Futoshi Hasebe, Kouichi Morita. Development
of a rapid and comprehensive proteomics-based arboviruses detection system.
J. Viro. Meth. Vol.167:31-36. 2010.
Nabeshima T, and Morita K.
Phylogeographic analysis of the migration of Japanese encephalitis virus in Asia.
Future Virology. Vol.5(3):343-351. 2010.
Kubo T., Agoh M, Mai le Q, Fukushima K, Nishimura H, Yamaguchi A, Hirano M, Yoshikawa A, Hasebe F, Kohno S, Morita K.
Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of panademic (H1N1) 2009 virus
as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings.
J Clin Microbiol. Vol.48(3):728-35. 2010.
Inoue S., Alonzo M., Kurosawa Y., Reyes J., Dimaano E., Alera M., Saito M., Oishi K., Hasebe F.,
Matias R., Natividad F., and Morita K.
Evaluation of a dengue IgG-indirect ELISA and a Japanese encephalitis IgG-indirect ELISA for diagnosis of
secondary dengue virus infection.
Vector-Borne and Zoonotic Diseases Vol.10(2):143-150. 2010.
Daisuke Hayasaka, et al.
Early Mortality following Intracerebral Infection with the Oshima strain of Tick-Borne Envephalitis Virus in a Mouse Model.
Journal of Veterinary Medical Science Vol.72(4):391-396. 2010.
Our department
Laboratory work
Structural-functional analyses
Development of detection drugs for SARS virus
For inquiries contact us.
Department of Virology
Institute of Tropical Medicine
Nagasaki University
1-12-4 Sakamoto
Nagasaki 852-8523
Japan
tel 81-95-819-7829
fax 81-95-819-7830
Institute of tropical medicine,
Nagasaki university.