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研究テーマ (熱帯性ウイルス疾患の予防、診断、治療に関する研究)

デングウイルス研究

ウエストナイルウイルス研究

日本脳炎ウイルス研究

SARSウイルス研究

ニパウイルス研究

研究トピック

西ナイルウイルス遺伝子検出系を実用化。(2004年7月28日) 栄研化学プレスリリース(PDAファイル、26KB)

新開発西ナイル熱ワクチンの有効性を確認。(2003年8月) 詳しくはこちら。

SARSウイルス検出試薬の開発を開始。(2003年6月) 詳しくはこちら。

主な論文

2015

Hayasaka D, Shimada S, Aoki K, Takamatsu Y, Uchida L, Horio M, Fuxun Y, Morita K. Epidemiological survey of severe fever with thrombocytopenia syndrome virus in ticks in Nagasaki, Japan. Trop Med Health. Accepted

Shimada S, Posadas-Herrera G, Aoki K, Morita K, Hayasaka D. Therapeutic effect of post-exposure treatment with antiserum on severe fever with thrombocytopenia syndrome (SFTS) in a mouse model of SFTS virus infection. Virology. 2015 Mar 25;482:19-27.

Takamatsu Y, Morita K, Hayasaka D. A unique amino acid substitution in NS2A protein of Japanese encephalitis virus affects virus propagation in vitro but not in vivo. J Virol. 2015 Mar 18.

Nagata N, Iwata-Yoshikawa N, Hayasaka D, Sato Y, Kojima A, Kariwa H, Takashima I, Takasaki T, Kurane I, Sata T, Hasegawa H. The pathogenesis of 3 neurotropic flaviviruses in a mouse model depends on the route of neuroinvasion after viremia. J Neuropathol Exp Neurol. 2015 Mar;74(3):250-60.

Yuki Takamatsu, Takeshi Nabeshima, Thuy Thi Thu Nguyen, Dinh Thi Dang, Ly Hoai Linh Pham, Hang Thi Pham, Lien Thi Nam Nguyen, Huong Ly Minh Phu, Thuy Thu Bui, Mai Thi Quynh Le, Kouichi Morita, Futoshi Hasebe. A Dengue virus serotype 4-dominated outbreak in central Vietnam, 2013. Journal of Clinical Virology. Vol.66, 24-26, 2015

Kyaw Zin Thant, Mya Myat Ngwe Tun, Maria del Carmen Parquet, Shingo Inoue, Yee Yee Lwin, Sanda Lin, Kay Thi Aye, Pe Thet Khin, Tin Myint, Khin Htwe, Takeshi Nabeshima and Kouichi Morita. Molecular Epidemiology of Dengue Viruses Co-circulating in Upper Myanmar in 2006. Tropical Medicine and Health Vol. 43, 21-27, 2015

Shirai K, Hayasaka D, Kitaura K, Takasaki T, Morita K, Suzuki R, Kurane I. Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus. Arch Virol. Vol.160(3):765-775, 2015

2014

Watanabe Y, Ito T, Ibrahim MS, Arai Y, Hotta K, Phuong HV, Hang NL, Mai LQ, Soda K, Yamaoka M, Poetranto ED, Wulandari L, Hiramatsu H, Daidoji T, Kubota-Koketsu R, Sriwilaijaroen N, Nakaya T, Okuno Y, Takahashi T, Suzuki T, Ito T, Hotta H, Yamashiro T, Hayashi T, Morita K, Ikuta K, Suzuki Y. A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity. Biosens Bioelectron. Vol.65C:211-219. 2014

Leo Uchida, Lyre Anni Espada-Murao, Yuki Takamatsu, Kenta Okamoto, Daisuke Hayasaka, Fuxun Yu, Takeshi Nabeshima, Corazon C. Buerano, and Kouichi Morita. The dengue virus conceals double-stranded RNA in the intracellular membrane to escape from an interferon response. Scientific Reports. 4:7395. doi: 10.1038/srep07395. 2014

Bijaya Gaire, Komal Raj Rijal, Biswas Neupane, Pravin Paudyal, Ishan Gautam, Megha Raj Banjara, Kouichi Morita and Basu Dev Pandey. Prevalence of dengue vector in relation to dengue virus infection in central region of Nepal. Dengue Bulletin.Vol.38:96-107, 2014

C. Murch, J.Lindenmayer, K. Pandey, G. Kaufman, D. D. Joshi, K. Morita , B. D.Pandey, EVALUATING THE USE OF EQUINE RABIES IMMUNOGLOBULIN (EQUIRAB) FOR POST EXPOSURE PROPHYLAXIS IN KATHMANDU, NEPAL, Nepalese Vet. J. 31:88-95, 2014

Takeshi Nabeshima, Shingo Inoue, Kenta Okamoto, Guillermo Posadas-Herrera, Fuxun Yu, Leo Uchida, Akitoyo Ichinose, Miako Sakaguchi, Toshihiko Sunahara, Corazon C. Buerano, Florencio P. Tadena, Ildefonso B. Orbita, Filipinas F. Natividad, Kouichi Morita. Tanay virus, a new species of virus isolated from mosquitoes in the Philippines. Journal of General Virology, Vol.95:1390-5. 2014

Aoki K, Shimada S, Simantini DS, Ngwe Tun MM, Buerano CC, Morita K, Hayasaka D. Type-I interferon response affects an inoculation dose-independent mortality in mice following Japanese encephalitis virus infection. Virol J. 11; 105, 2014

Ngwe Tun MM, Aoki K, Senba M, Buerano CC, Shirai K, Suzuki R, Morita K, Hayasaka D. Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus. Sci Rep. 4:5344, 2014

Ngwe Tun MM, Thant KZ, Inoue S, Nabeshima T, Aoki K, Kyaw AK, Myint T, Tar T, Maung KTT, Hayasaka D, Morita K. Emergence of East Central South African Genotype of Chikungunya Virus in Myanmar, 2010. Emerg Infect Dis. 20:1378-1381, 2014

Tomoko Abe, Ayumi Sando, Fumiteru Teraoka, Tadamune Otsubo, Kouichi Morita, Hiroaki Tokiwa , Kiyoshi Ikeda , Takashi Suzuki , Kazuya I.P.J. Hidari. Computational design of a sulfoglucuronide derivative fitting into a hydrophobic pocket of dengue virus E protein. Biochemical and Biophysical Research Communications. Vol449:32-37, 2014

Le Xuan Luat, Mya Myat Ngwe Tun, Corazon C. Buerano, Kotaro Aoki, Kouichi Morita and Daisuke Hayasaka. Pathologic potential of variant clones of the Oshima strain of Far Eastern subtype tick-borne encephalitis virus. Tropical Medicine and Health. Vol. 42:15-23, 2014

Fumihiko Yasui, Michinori Kohara , Masahiro Kitabatake , Tetsu Nishiwaki , Hideki Fujii, Chise Tateno, Misako Yoneda, Kouichi Morita, Kouji Matsushima , Shigeo Koyasu, Chieko Kai. Phagocytic cells contribute to the antibody-mediated elimination of pulmonary-infected SARS coronavirus. Virology 454-455: 157-168. 2014

Muhareva Raekiansyah, Lyre Anni Espada-Murao, Kenta Okamoto, Toru Kubo, Kouichi Morita . Dengue virus neither directly mediates hyperpermeability nor enhances TNF-α-induced permeability in vitro. Japanese Journal of Infectious Diseases, Vol.67:86-94, 2014

Yuki Takamatsu, Kenta Okamoto, Duc Tuan Dinh, Fuxun Yu, Daisuke Hayasaka, Leo Uchida, Takeshi Nabeshima, Corazon C. Buerano and Kouichi Morita, NS1’ protein expression facilitates production of Japanese encephalitis virus in avian cells and embryonated chicken eggs, Journal of General Virology, 95, 373-383. 2014

2013

Biswas Neupane, Komal Raj Rijal, Gyan Bahadur Aryal,Yogendra Shah, Megha Raj Banjara, Jeevan Bahadur Sherchand, Kouichi Morita and Basu Dev Pandey. Clinical and laboratory features of dengue fever in the southern lowlands of Nepal, Dengue Bulletin, Vol.37:1-10, 2013

Haorile Chagan-Yasutan, Lishomwa C. Ndhlovu, Talitha Lea Lacuesta, Toru Kubo, Prisca Susan A. Leano, Toshiro Niki, Shigeru Oguma, Kouichi Morita, Glen. M. Chew, Jason D. Barbour, Elizabeth Freda O. Telan, Mitsuomi Hirashima, Toshio Hattori, Efren M. Dimaano. Galectin-9 Plasma Levels Reflect Adverse Hamatological and Immunological Features in Acute Dengue Virus Infection J.Clin.Virol. Vol.58. 635-640, 2013

Khalid A Enan, Takeshi Nabeshima, Toru Kubo, Corazon C Buerano, Abdel Rahim M . El Hussein, Isam M Elkhidir, Eltahir AG Khalil and Kouichi Morita. Survey of causative agents for acute respiratory infections among patients in Khartoum- State, Sudan, 2010- 2011. Virology Journal, 10:312. 2013

Yuki Takamatsu, Leo Uchida, Phan Thi Nga, Kenta Okamoto, Takeshi Nabeshima, Dang Thi Thu Thao, Do Thien Hai, Nguyen Thi Tuyet, Hoang Minh Duc, Le Xuan Luat, Futoshi Hasebe and Kouichi Morita. An approach for differentiating Echovirus 30 and Japanese encephalitis virus infections in acute meningitis/encephalitis: a retrospective study of 103 cases in Vietnam. Virology Journal 10:280, 2013

Basu D. Pandey, Takeshi Nabeshima, Kishor Pandey, Saroj P. Rajendra, Yogendra Shah, Bal R. Adhikari, Govinda Gupta, Ishan Gautam, Mya M. N. Tun, Reo Uchida, Ichiro Kurane and Kouichi Morita. First Isolation of Dengue Virus from the 2010 Epidemic in Nepal. Tropical Medicine and Health Vol. 41 No. 3: 1-9, 2013

Nguyen Thanh Thuy, Tran Quang Huy, Phan Thi Nga, Kouichi Morita, Irene Dunia, Lucio Benedetti. A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line. Virology Vol.444, 337-342, 2013

Chris Lauber, Jelle J. Goeman, Maria del Carmen Parquet, Phan Thi Nga4, Eric J. Snijder1, Kouichi Morita, Alexander E. Gorbalenya, The Footprint of Genome Architecture in the Largest Genome Expansion in RNA Viruses. PLoS Pathogens 9 (7): e1003500., July 2013

Hayasaka D, Shirai K, Aoki K, Nagata N, Simantini DS, Kitaura K, Takamatsu Y, Gould E, Suzuki R, Morita K. TNF-α acts as an immunoregulator in the mouse brain by reducing the incidence of severe disease following Japanese encephalitis virus infection. PLoS One. August 5;8(8):e71643. 2013

Hayasaka D, Aoki K, Morita K. Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop -mediated isothermal amplification. Virology Journal Mar 4;10:68. 2013

Ngwe Tun MM, Thant KZ, Inoue S, Kurosawa Y, Lwin YY, Lin S, Aye KT, Thet Khin P, Myint T, Htwe K, Mapua CA, Natividad FF, Hirayama K, Morita K. Serological characterization of dengue virus infections observed among dengue hemorrhagic fever/dengue shock syndrome cases in Upper Myanmar. J.Med.Virol. Vol.85:1258-1266, 2013

Kwallah AO, Inoue S, Muigai AW, Kubo T, Sang R, Morita K, Mwau M., A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus. J Virol Methods. (May 18) ;Vol.193(1):23-27. 2013

2012

Hidari KI, Ikeda K, Watanabe I, Abe T, Sando A, Itoh Y, Tokiwa H, Morita K, Suzuki. T 3-O-sulfated glucuronide derivative as a potential anti-dengue virus agent. Biochem Biophys Res Commun. 2012 Aug 3;424(3):573-8.

Lauber C, Ziebuhr J, Junglen S, Drosten C, Zirkel F, Nga PT, Morita K, Snijder EJ, Gorbalenya AE. Mesoniviridae: a proposed new family in the order Nidovirales formed by a single species of mosquito-borne viruses. Arch Virol. Vol.157, 1623-1628. 2012

Kenta Okamoto, Hitomi Kinoshita, Maria del Carmen Parquet, Muhareva Raekiansyah, Daisuke Kimura, Katsuyuki Yui, Mohammed Alimul Islam, Futoshi Hasebe, Kouichi Morita. Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. Journal of General Virology. Vol. 93(4): 761-770. 2012

Futoshi Hasebe, Nguyen Thi Thu Thuy, Shingo Inoue, Fuxun Yu, Yoshihiro Kaku, Shumpei Watanabe, Hiroomi Akashi, Dang Tuan Dat, Le Thi Quynh Mai, Kouichi Morita. Serologic Evidence of Nipah Virus Infection in Bats, Vietnam. Emerging Infectious Diseases. Vol.18(3):536-7. 2012

Takahisa Furuta, Lyre Anni Murao, Nguyen Thi Phuong Lan, Nguyen Tien Huy, Vu Thi Que Huong, Tran Thi Thuy, Vo Dinh Tham, Cao Thi Phi Nga, Tran Thi Ngoc Ha, Yasukazu Ohmoto, Mihoko Kikuchi, Kouichi Morita, MichioYasunami, Kenji Hirayama, and Naohiro Watanabe, Association of Mast Cell-Derived VEGF and Proteases in Dengue Shock Syndrome. PLoSNTD, Vol.6:e1505.2012

2011

Tran Thi Ngoc Ha, Nguyen Tien Huy, Lyre Anni Murao, Nguyen Thi Phuong Lan, Tran Thi Thuy, Ha Manh Tuan, Cao Thi Phi Nga, Vo Van Tuong, Tran Van Dat, Mihoko Kikuchi, Michio Yasunami, Kouichi Morita, Vu Thi Que Huong, Kenji Hirayama. Elevated Levels of Cell-Free Circulating DNA in Patients with Acute Dengue Virus Infection. Plos One 6(10):e25969. 2011

Phan Thi Nga, Maria del Carmen Parquet, Chris Lauber, Manmohan Parida, Takeshi Nabeshima, Fuxun Yu, Nguyen Thanh Thuy, Shingo Inoue, Takashi Ito, Kenta Okamoto, Akitoyo Ichinose, Eric J. Snijder, Kouichi Morita, Alexander E. Gorbalenya. Discovery of the First Insect Nidovirus, a Missing Evolutionary Link in the Emergence of the Largest RNA Virus Genomes. PLoS Pathogens 7 (9): e1002215. 2011

Lyre Anni Espada-Murao and Kouichi Morita. Delayed cytosolic exposure of Japanese encephalitis virus double-stranded RNA impedes interferon activation and enhances viral dissemination in porcine cells. Journal of Virology,Vol.85(13):6736-6749, 2011

Duc Tuan Dinh, Mai Thi Quynh Le, Cuong Duc Vuong, Futoshi Hasebe and Kouichi Morita. An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses. Tropical Medicine and Health,Vol.39(1):3-7, 2011

Wichit S, Jittmittraphap A, Hidari KI, Thaisomboonsuk B, Petmitr S, Ubol S, Aoki C, Itonori S, Morita K, Suzuki T, Suzuki Y, Jampangern W. Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito calls. Microbiol Immunol. Vol. 55(2) : 135-140,2011

2010

Yasui F., Kai C., Saito K., Inoue S., Yoneda M., Morita K., Mizuno K., Kohara M. Analysis of the mechanism by which BALB/c mice having prior immunization with nucleocapsid protein of SARS-CoV develop severe pneumonia after SARS-CoV
infection.
Procedia in Vaccinology. Vol.2:42-48, 2010

Michael O Baclig, Leonora TS Gervacio, Lady-Anne C Suarez, Corazon C Buerano, Ronald R Matias, Atsushi Kumatori, Shingo Inoue, Kouichi Morita, Filipinas F Natividad and Futoshi Hasebe. Flow Cytometric Analysis of Dengue Virus-Infected Cells in Peripheral Blood. Southeast Asian J Trop Med Public Health, Vol.41(6): 1352-1358, 2010

Guillermo Posadas-Herrera, Shingo Inoue, Isao Fuke, Yuko Muraki, Cynthia A. Mapua, Afjal Hossain Khan, Maria del Carmen Parquet, Sadao Manabe, Osamu Tanishita, Toyokazu Ishikawa, Filipinas F. Natividad, Yoshinobu Okuno, Futoshi Hasebe, Kouichi Morita. Development and evaluation of a formalin-inactivated West Nile Virus vaccine (WN-VAX) for a human vaccine candidate. Vaccine 28, 7939-7946, 2010

Daisuke Katoa, Shota Era, Ippei Watanabe, Masataka Arihara, Nobuo Sugiura, Koji Kimata, Yasuo Suzuki, Kouichi Morita, Kazuya I.P.J. Hidari, Takashi Suzuki. Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein. Antiviral Research, Vol.88:236-243 2010.

Kouichi Morita. West Nile fever: Expected vaccine for the future, Clinical Inspection, Vol.54:1411-1446, 2010

Kenta Okamoto, Yushirou Endo, Shingo Inoue, Takeshi Nabeshima, Phan Thi Nga, Posadas H. Guillermo, Fuxun Yu, Do Phuong Loan, Bui Minh Trang, Filipinas F. Natividad, Futoshi Hasebe, Kouichi Morita. Development of a rapid and comprehensive proteomics-based arboviruses detection system. J. Virol. Meth. Vol.167:31-36. 2010.

Nabeshima T, and Morita K. Phylogeographic analysis of the migration of Japanese encephalitis virus in Asia. Future Virology.Vol5:343-351 2010.

Kubo T., Agoh M, Mai le Q, Fukushima K, Nishimura H, Yamaguchi A, Hirano M, Yoshikawa A, Hasebe F, Kohno S, Morita K. Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of pandemic (H1N1) 2009 virus as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings. J Clin Microbiol. Vol.48(3):728-35. 2010.

Inoue S., Alonzo M., Kurosawa Y., Reyes J., Dimaano E., Alera M., Saito M., Oishi K., Hasebe F., Matias R., Natividad F., and Morita K. Evaluation of a dengue IgG-indirect ELISA and a Japanese encephalitis IgG-indirect ELISA for diagnosis of secondary dengue virus infection. Vector-Borne and Zoonotic Diseases. Vol.10(2): 143-150, 2010.

Daisuke Hayasaka, et al. Early Mortality following Intracerebral Infection with the Oshima strain of Tick-Borne Encephalitis Virus in a Mouse Model. Journal of Veterinary Medical Science. 72(4):391-396 2010.

2009

Kouichi Morita Molecular epidemiology of Japanese encephalitis in East Asia. Vaccine. Vol.27, 7131-7132, 2009.

Hitomi Kinoshita, Edward Gitau Matumbi Mathenge, Nguyen Thanh Hung, Vu Thi Que Huoung, Atsushi Kumatori, Fuxun Yu, Maria del Carmen Parquet, Shingo Inoue, Ronald Roll Matias, Filipinas Florendo Natividad, Kouichi Morita, and Futoshi Hasebe. Isolation and Characterization of Two Phenotypically Distinct Dengue Type-2 Virus Isolates from the Same Dengue Hemorrhagic Fever Patient.. Japanese Journal of Infectious Diseases. Vol.62, No.5, 343-350, 2009.

Daisuke Hayasaka, et al. Mortality following peripheral infection with tick-borne encephalitis virus results from a combination of central nervous system pathology, systemic inflammatory and stress responses. Virology. 390(1):139-150 2009.

Shoko Honda, Mariko Saito, Efren M. Dimaano, Philip A. Morales, Maria T.G. Alonzo Lady-Anne C. Suarez, Natsuki Koike, Shingo Inoue, Atsushi Kumatori, Ronald R. Matias, Filipinas F. Natividad, and Kazunori Oishi. Increased Phagovytosis of Platelets from Patients with Secondary Dengue Virus Infection by Human Macrophages. Am. J. Trop. Med. Hyg. Vol.80(5), 841-845, 2009.

Dong Tu Nguyen, Tuan Cuong Ngo, Huy Hoang Tran, Thi Phuong Lan Nguyen, Binh Minh Nguyen, Kouichi Morita and Masahiko Ehara. Two different mechanisms of ampicillin resistance operating in strains of Vibrio cholerae 01 independent of resistance genes. FEMS Microbiology Letters. Vol.298, 31-43, 2009.

Takeshi Nabeshima, Hyunh Thi Kim Loan, Shingo Inoue, Makoto Sumiyoshi, Yasuhiro Haruta, Phan Thi Nga, Vu Thi Que Huoung, Maria del Carmen Parquet, Futoshi Hasebe and Kouichi Morita, Evidence of frequent introductions of Japanese encephalitis virus from south-east Asia and continental east Asia to Japan. Journal of General Virology. Vol.90, 827-832, 2009.

Le Roux CA, Kubo T, Grobbelaar AA, van Vuren PJ, Weyer J, Nel LH, Swanepoel R, Morita K, Paweska JT. Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens. J Clin Microbiol. Mar; 47(3):645-51. 2009.

2008

Manmohan Parida, Santosh Sannarangaiah, Paban Kumar Dash, P.V.L. Rao and Kouichi Morita, Loop mediated isothermal amplification(LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Reviews in Medical Virology, Vol.18, 407-422, 2008.


Nguyen Thi Phuong Lan, Mihoko Kikuchi, Vu Thi Que Huong, Do Quang Ha, Tran Thi Thuy, Vo Dinh Tham, Ha Manh Tuan, Vo Van Tuong, Cao Thi Phi Nga, Tran Van Dat, Toshifumi Oyama, Kouichi Morita, Michio Yasunami, Kenji Hirayama. Protective and Enhancing HLA Alleles, HLA-DRB1*0901 and HLA-A*24, for Severe Forms of Dengue Virus Infection, Dengue Hemorrhagic Fever and Dengue Shock Syndrome. PLoS Neglected Tropical Diseases, Vol. 2. e304, 2008.

Kazuya I.P.J. Hidari, Naonori Takahashi, Masataka Arihara, Masato Nagaoka, Kouichi Morita, Takashi Suzuki Structure and anti-denque virus activity of sulfated polysaccharide from a marine alga . Biochemical and Biophysical Research Communications, 376, 91-95; 2008.

Basu Dev Pandey, Kouichi Morita, Santa Raj Khanal, Tomohiko Takasaki, Isao Miyazaki, Tetsuro Ogawa, Shingo Inoue, Ichio Kurane, Dengue virus, Nepal. . Emerging Infectious Diseases Vol. 14(3), 514-515; 2008.

Takeshi Nabeshima, Phan Thi Nga, Posadas Guillermo, Maria del Carmen Parquet, Fuxun Yu, Nguyen Thanh Thuy, Bui Minh Trang, Nguyen Tran Hien, Vu Sinh Nam, Shingo Inoue, Futoshi Hasebe and Kouichi Morita. Isolation and Molecular Characterization of Banna Virus from Mosquitoes, Vietnam. . Emerging Infectious Diseases Vol. 14(8), 1276-1279; 2008.

Yasui, F., Kai, C., Kitabatake, M., Inoue, S., Yoneda, M., Yokochi, S., Kase, R., Sekiguchi, S., Morita, K., Hishima, T., Suzuki, H., Karamatsu, K., Yasutomi, Y., Shida, H., Kidokoro, M., Misuno, K., Matsushima, K., Kohara, M.. Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV. . J Immunol. Vol.181(9), 6337-48; 2008.

Liu, J., Liu, B., Cao, Z., Inoue, S., Morita, K., Tian, K., Zhu, Q., Gao, G.F., Characterization and application of monoclonal antibodies specific to West Nile virus envelope protein. . J Virol Methods. Vol. 154(1-2):20-6, 2008.

2007

Fukun Yu, Futoshi Hasebe, Shingo Inoue, Edward G. Mathenge and Kouichi Morita, Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein. . Arch Virol. Vol.152: 1859-1869, 2007.

Parida, M.M., Santhosh S.R., Dash, P.K., Tripathi, N.K., Lakshmi, V., Mamidi, Ambuj, N., Gupta, N., Saxena, P., Pradeep Babu, J., Lakshmana Rao P.V., and Morita, K. Rapid and Real-time detection of Chikungunya virus by Reverse Transcription Loop Mediated Isothermal Amplification (RT-LAMP) Assay. . .J.Clin. Microbiol. Vol.45, 351-357, 2007.

Fukun Yu, Mai Quynh Le Thi, Shingo Inoue, Futoshi Hasebe, Maria del Carmen Parquet, Shigeru Morikawa and Kouichi Morita, Recombinant Truncated Nucleocapsid Protein as Antigen in a Novel Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Severe Acute Respiratory Syndrome Coronavirus Infection. . Clinical and Vaccine Immunology Vol.14, 146-149, 2007.

Masahiro Kitabatake., Shingo Inoue, Fumihiko Yasui, Shoji Yokochi, Masaaki Arai, Kouichi Morita, Hisatoshi Shida, Minoru Kidokoro, Fukashi Murai, Mai Quynh Le Thi, Kyosuke Mizunoi, Kouji Matsushima, Michinori Kohara. SARS-CoV spike protein-expressing recombinant vaccinia virus efficiently induces neutralizing antibodies in rabbits pre-immunized with vaccinia virus . . .Vaccine Vol.25, 630-637, 2007.

Yuuki Inoue, Nobuyuki Tanaka, Yoshinori Tanaka, Shingo Inoue, Kouichi Morita, Min Zhuang, Toshio Hattori and Kazuo Sugamura. Clathrin-Dependent Entry of Severe Acute Respiratory Syndrome Coronavirus into Target Cells Expressing ACE2 with the Cytoplasmic Tail Deleted. . J Virol. Vol.181, 8722-8729, 2007.

Ito M, Yadama K, Takasaki T, Pandey B, Nerome R, Tajima S, Morita K, Kurane I. Phylogenetic analysis of dengue viruses isolated from imported dengue patients: possible aid for determining the countries where infections occurred. . J Travel Med. Vol.14(4), 233-44, 2007.

2006

Parida, M.M., Santhosh S.R., Dash, P.K., Tripathi, Saxena, N.K., Ambuj, P.S., Sahni, A.K., Rao, P.V., Lakshmana and Morita, K. Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of Japanese Encephalitis Virus. . .J.Clin. Microbiol. Vol.44, 4172-4178, November 2006.

Fukun Yu, Nor Shahidah Khairullah, Shingo Inoue, Vijayamalar Balasubramaniam, Stella Joan Berendam, Leok Kin Teh, Nik Shamsiah Wan Ibrahim, Sohayati Abdul Rahman, Sharifah Syed Hassan, Futoshi Hasebe, Mangalam Shinniah, and Kouichi Morita, Serodiagnosis Using Recombinant Nipah Virus Nucleocapsid Protein Expressed in Escherichia coli. . .J.Clin. Microbiol. Vol.44, 3134-3138, Sept. 2006.

Aoki C., Hidari, K.I.P.J, Itonori, S., Yamada, A., Takahashi, N., Kasama, T., Hasebe, F., Islam, M.A., Hatano, K., Matsuoka, K., Taki, T., Guo, C.T., Takahashi, T., Sakano, Y., Suzuki, T., Miyamoto, D., Sugita, M., Terunuma, D., Morita, K., and Suzuki, Y. Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type2. . .Journal of Biochemistry Vol.139, 607-614, 2006.

Islam M.A., Ahmed M.U., Begum N., Chowdhury N.A., Khan A.F., Parquet M.C, Bipolo S., Inoue S., Hasebe F., Suzuki Y., and Morita, K., Molecular Characterization and Clinical Evaluation of Denque Outbreak in 2002 in Bangladesh. . .Japanese J. Infectious Diseases. Vol.59: 85-91, 2006.

Paresh Sumatilal Shah, Mariko Tanaka, Afjal Hossain Khan, Edward Gitau Matumbi Mathenge, Isao Fuke, Mitsuo Takagi, Akira Igarashi, and Kouichi Morita., Molecular Characterization of Attenuated Japanese Encephalitis live Vaccine Strain ML-17. . .Vaccine Vol.24: 402-411, 2006.

Kanzawa, N., Nishigaki, K., Hayashi, T., Ishii, Y., Furukawa, S., Niiro A., Yasui, F., Kohara, M., Morita, K., Matsushima, K., Le M.Q., Masuda, T., and Kannagi, M., Augmentation of chemokine production by severe acute respiratory syndrome coronavirus 3a/X1 and 7a/X4 proteins through NF-kB activation. . .FEBS Letters, Vol.22: 6807-6812, December 2006.

Kazunori Oishi, Cynthia A. Mapua, Celia C. Carlos, Maria T.D.D. Cinco-Abanes, Mariko Saito, Shingo Inoue, Kouichi Morita and Filipinas F. Natividad Dengue and other Febrile illnesses among Children in the Philippines . Dengue Bulletin - Volume 30: 26-34, December 2006.

2005

Leonora T.D. Salda, Maria D.C.Parquet, Ronald R. Matias, Filipinas F. Natividad, Nobuyuki Kobayashi, Kouichi Morita. Molecular Epidemiology of dengue 2 viruses in the Philippines: Genotype shift and local evolution. Am.J.Trop.Med.Hyg. Vol.73, 796-802, 2005.

Nawa M., Takasaki T., Ito M., Inoue S., Morita K., and Kurane I. Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Sero diagnosis of Dengue Virus Infection. Clinical and Diagnostic Laboratory Immunology. Vol.12, 1235-1237, 2005.

Manmohan Parida, Kouhei Horioke, Hiroyuki Ishida, Paban Kumar Dash, Parag Saxena, Asha Mukul Jana, Mohammed Alimul Islam, Shingo Inoue, Norimitsu Hosaka, and Kouichi Morita.Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay. J. Clin. Microbiol. Vol.43: 2895-2903, 2005.

Wei-Feng Tang, Yuki Eshita, Masayuki Tadano, Kouichi Morita and Yoshihiro Makino. Molecular basis for adaptation of a chimeric dengue type4/Japanese encephalitis virus to vero cells. Microbiol. Immunol., Vol.49:285-294, 2005.

Celia C. Carlos, Kazunori Oishi,* Maria T. D. D. Cinco, Cynthia A. Mapua, Shingo Inoue, Deu John M. Cruz, Mary Ann M. Pancho, Carol Z. Tanig, Ronald R. Matias, Kouichi Morita, Filipinas F. Natividad, Akira Igarashi, And Tsuyoshi Nagatake. Comparison of Clinical Features and Hematologic Abnormalities Between Dengue Fever and Dengue Hemorrhagic Fever Among Children in the Philippines. Am. J. Trop. Med. Hyg., Vol.73, 435-440,2005.

Fuxun Yu, Mai Quynh Le, Shingo Inoue, Hong Thi Cam Thai, Futoshi Hasebe, Maria del Carmen Parquet, Kouichi Morita. Evaluation of Inapparent Nosocomial Server Acute Respiratory Syndrome Coronavirus Infection in Vietnam by use of Highly Specific Recombinant Truncated Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay. Clinical and Diagnostic Laboratory Immunology. Vol.12, 848-854,2005.

2004

Edward Gitau Matumbi Mathenge, Maria del Carmen Parquet, Yasutomo Funakoshi, Seiji Houhara, Pooi Fong Wong, Akitoyo Ichinose, Futoshi Hasebe, Shingo Inoue, Kouichi Morita. "Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice." Journal of General Virology. (Vol.85: 2503-2513, 2004)

Nga PT., Parquet MC, Cuong VD, Ma S-P., Hasebe F, Inoue S, Makino Y, Takagi M, Nam VS and Morita K. "Shift in JEV genotype circulating in northern Vietnam: Implication for frequent introductions of JEV from Southeast Asia to East Asia." Journal of General Virology. Vol.85: 1625-1631, 2004

Hong Thi Cam Thai, Mai Quynh Le, Cuong Duc Vuong, Manmohan Parida, Harumi Minekawa, Tsugunori Notomi, Futoshi Hasebe, and Kouichi Morita. "Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus.". J.Clin.Microbiol. Vol.42: 1956-1961, 2004.

Parida M., Posadas M., Inoue S., Hasebe F., and Morita K.. "Real-time reverse transcription loop mediated isothermal amplification for rapid detection of West Nile virus." J.Clin.Microbiol. Vol.42: 257-263, 2004.

2003

Pandey B., Yamamoto A., Morita K., Kurosawa Y., Rai S., Adhikari S., Kandel P., and Kurane I.: Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay. Epidemiol.Infect. 131:881-885,2003.

Oishi K., Inoue A., Kuramoto T., Onizuka S., Saito M., Hasebe F., Morita K., Nagatake T.: Association of dengue virus type-specific IGG on platelets is specific for the acute phase in an imported Japanese patient with seconday dengue virus infection. J.J.Trop.Med.Hygn. Vol.31:223-225, 2003.

Inoue S., Morita K., Matias R.R., Tuplano J.V., Resuello R.R.G., Candelario J.R., Cruz D.J.M., Mapua C.A., Hasebe F., Igarashi A. and Natividad F.F. Distribution of three arbovirus antibodies among monkeys (Macaca facscicularis) in the Philippines. J.Med.Primatol. 32:89-94, 2003

Ohishi K., Inoue S., Cinco M., Diaano E., Alera M., Alfon J., Abanes F., Crus D., Matias R., Matsuura H., Hasebe F., Tanimura S., Kumatori A., Moirta K., Natividad F., Nagatake T: Correlation between increased platelet-associated IgG and thronbocytopecia in secondary dengeu virus infections. J.Med.Virol. 71:259-264, 2003

2002

Afjal Hossain Khan, Kouichi Morita, Maria del Carmen Parquet, Futoshi Hasebe,Edward G. M. Mathenge and Akira Igarash. Complete Nucleotide Sequence of Chikungunya Virus and Evidence of an Internal Polyadenylation site. J.Gen.Virol 83:3075-3084, 2002

Pandey B.D., Thapa L.B., Sherchand J.B., Rimal N., Bhattarai A., and Morita K. Etiology of diarrhoea among adult patients during the early monsoon period in Kathmandu, Nepal. J.J.Trop.Med.and Hygiene. 30:133-137. 2002.

Parquet M.C., Kumatori A., Hasebe F., Edward G.M. Mathenge E.G.M.and Morita K. Saint Louis Encephalitis Virus induced pathology in culture cells. Archives of Virology 147:1105-1119. 2002

Hasebe F., Parquet M.C., Pandey B. D, Mathenge E.G.M., Morita K., V. Balasubramaniam V., Saat Z., Yusop A., Sinniah, M., Natkunam S. and Igarashi K.Combined Detection and Genotyping of Chikungunya Virus by a Specific Reverse Transcription-Polymerase Chain Reaction. J.Med.Virol. 67:370-374. 2002

Vargas R.E.M., Morita K., Eshita Y., Tsuda Y., Fukuma T. and Takagi M. Infection and dissemination of two dengue type 2 viruses isolated from patients exhibiting different disease severity in orally infected Aedes aegypti from different geographic origin. Journal of Medical Entomology and Zoology 53:21-27. 2002

2001

Morita K., Tadano M., Nakaji S., Kosai K., Mathenge E.G.M., Pandey B.D., Hasebe F., Inoue S., and Igarashi A. Locus of a virus neutralization epitope on the Japanese encephalitis virus envelope protein determined by use of Long PCR-based region-specific random mutagenesis. Virology 287:417-426, 2001.

Parquet M.C., Kumatori A., Hasebe F., Morita K. and Igarashi A. West Nile Virus induced bax-dependent apoptosis. FEBS Letters 500:17-24, 2001.

 

 

実験

熱帯ウイルスの分子生物学的研究

SARSウイルス検出試薬の開発

開発内容の詳細はこちらでごらんになれます。


デングウイルス

デング熱はデングウイルス(フラビウイルス属で血清型が1〜4 型まである) によって引き起こされる感染症で、通常5〜6 日の潜伏期を経て、突然の発熱で はじまり、激しい頭痛、関節痛、筋肉痛、発疹を伴います。ウイルスを保有して いる蚊(ネッタイシマカ、ヒトスジシマカなど)に吸血されることにより感染し、 マラリアと同様にアジアや太平洋諸島など熱帯亜熱帯地域に広く分布していま す。デング熱を媒介する蚊は空き缶などに溜まった水や竹の切り株に溜まった水 でも発生するため都会で流行することも多く、流行する地域全体で年間数千万人 の感染者が発生しています。特に東南アジア、南アジア、中南米において多くの 患者が報告されています。

西ナイルウイルス

西ナイル熱は節足動物を介してヒトに伝播する感染症で、フラビウイルス属の 西ナイルウイルスによって引き起こされます。鳥と蚊の感染サイクルによって維 持されており、主として蚊を介してヒトに感染し、発熱や脳炎を引き起こします。 日本での感染例は認められていませんが、ヨーロッパやアメリカなどでは流行し ています。北米では感染鳥の発病や死亡、ウマとヒトにおける流行、重篤な脳炎 患者の発生が顕著で、新興感染症・輸入感染症として注意が必要な疾患です。

日本脳炎ウイルス

日本脳炎は、主にコガタアカイエカによって媒介される日本脳炎ウイルスに よっておこるウイルス感染症で、ヒトに重篤な急性脳炎をおこします。日本脳炎 ウイルスはフラビウイルス科に属するウイルスです。

 



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Copyright©2003-2005 Department of Virology,
Institute of tropical medicine,
Nagasaki university.