長崎大学 熱帯医学研究所 新興感染症学分野

長崎大学熱帯医学研究所
新興感染症学分野
〒852-8523
長崎県長崎市坂本1-12-4
TEL:095-819-7848
FAX:095-819-7851
  • 長崎大学
  • 長崎大学大学院 医歯薬学総合研究科
  • 長崎大学 熱帯医学研究所(熱研)
  • Tropical Medicine and Health

研究業績

原著論文

  1. Maehira Y., Jakimo A.L., Saito T., Kurosaki Y., Yasuda J., Tarui M., Malvy D.J.M, and Takeuchi T.: Responding to ever-changing epidemiological dynamics of Ebola virus disease. BMJ Global Health, in press.
  2. Kurosaki Y., Okada S., Nakamae S., and *Yasuda J.: A loop-mediated isothermal amplification assay for rapid and sensitive detection of bovine papular stomatitis virus. Journal of Virological Methods, 238, 42-47, 2016.
  3. Uchida L., Urata S., Ulanday G.E., Takamatsu Y., Yasuda J., Morita K., and Hayasaka D.: Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation. Viruses, 8 (2), E46 (1-12), 2016.
  4. Shimada S., Aoki K., Nabeshima T., Fuxun Y., Kurosaki Y., Shiogama K., Onouchi T., Sakaguchi M., Fuchigami T., Ono H., Nishi K., Posadas-Herrera G., Uchida L., Takamatsu Y., Yasuda J., Tsutsumi Y., Fujita H., Morita K., and Hayasaka D.: Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. Scientific Reports, 6:20213 (1-11), 2016.
  5. Kurosaki Y, Magassouba N, Bah HA, Soropogui B, Doré A, Kourouma F, Cherif MS, Keita S, Yasuda J:Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Test for Ebola Virus Surveillance in Remote Areas in Guinea. J. Infect Dis. 2016 (In press)
  6. Kurosaki Y, Okada S, Nakamae S, Yasuda J. Loop-mediated isothermal amplification assay for rapid and sensitive detection of bovine papular stomatitis virus. J Virol Methods. 2016 (in press)
  7. Kurosaki Y, Magassouba N, Oloniniyi OK, Cherif MS, Sakabe S, Takada A, Hirayama K, Yasuda J.:Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea. PLoS Negl Trop Dis. 2016 Feb 22;10(2):e0004472
  8. Urata S., Weyer J., Storm N., Miyazaki Y., van Vuren P.J., Paweska J.T., and Yasuda J.: Analysis of assembly and budding of Lujo virus. Journal of Virology 90(6):3257-61, 2016
  9. Chisato N. Yasuda J.: Roles of the three L-domains in β-retrovirus budding. Microbiology and Immunology. vol.59 (9), 545-54, 2015
  10. Urata S., Yasuda J.: Cis- and cell type-dependent trans-requirements for Lassa virus-like particle production. J Gen Virol. vol.96 (Pt7) 1626-35, 2015
  11. Yoshikawa R., Miyaho RN., Hashimoto A., Abe M., Yasuda J., Miyazawa T.: Suppression of production of baboon endogenous virus by dominant negative mutants of cellular factors involved in multivesicular body sorting pathway. Virus Res. vol. 196, 128-134, 2015
  12. Abe M., Fukuma A., Yoshikawa R., Miyazawa T., Yasuda J.: Inhibition of budding/release of porcine endogenous retrovirus. Microbiol Immunol. vol. 58 (8): 432-8, 2014
  13. Shimode S., Nakaoka R., Hoshino S., Abe M., Shogen H., Yasuda J., Miyazawa T.; Identification of cellular factors required for the budding of koala retrovirus. Microbiol. Immunol. vol. 57 (7), 543-6, 2013
  14. Shojima. T., Nakaoka, R., Hoshino, S., Abe, M., Shogen, H., Yasuda, J.., and Miyazawa, T.: Construction and characterization of an infectious molecular clone of koala retrovirus. Journal of Virology, 87(9), 5081-5088, 2013.
  15. Fukuma, A., Yoshikawa, R., Miyazawa, T., and *Yasuda, J.: A new approach to establish a cell line with reduced risk of endogenous retroviruses. PLoS One, 8(4), e61530 (1-5), 2013.
  16. Imakawa, K., Yasuda, J., Kobayashi, T., and Miyazawa, T.: Changes in Gene Expression Associated with Conceptus Implantation to the Maternal Endometrium. Journal of Mammalian Ova Research, 30, 2013. in press.
  17. Okada J., Horiuchi1, H., Hashimoto, K., Hirosawa, D., Kurosaki, Y., Kawamoto, K, Yasuda, J., Makino, S., Gemma, N., and Nikaido, M.: Mobile Automatic Detection System for Bacillus anthracis using Electrochemical DNA Chip. Journal of Biosensors and Bioelectronics, 3, 126, 2012.
  18. Takeda E., Nakagawa S., Nakaya Y., Tanaka A., Miyazawa T., Yasuda, J.: Identification and Functional Analysis of Three Isoforms of Bovine BST-2. PLoS one, 7, e41483, 2012.
  19. Urata S., Nhi N., de la Torre J. C.: The PI3K/Akt pathway contributes to Arenavirus budding. Journal of Virology, 86 (8), 4578-85, 2012.
  20. Fukuma A., Abe M., Urata S., Yoshikawa R., Morikawa Y., Miyazawa T., Yasuda, J.: Viral and cellular requirements for the budding of Feline Endogenous Retrovirus RD-114. Virology Journal, 8 (1), 540, 2011.
  21. Yoshikawa R., Yasuda, J., Kobayashi T., Miyazawa T.: Canine ASCT1 and ASCT2 are functional receptors for RD-114 virus in dogs. Journal of General Virology, 93, 603-7, 2012.
  22. Nagashima, S., Takahashi, M., Jirintai, S., Tanaka, T., Nishizawa, T., Yasuda, J., Okamoto, H.: Tsg101 and the vacuolar protein sorting pathway are required for release of hepatitis E virions. Journal of General Virology, 92, 2838-2848, 2011.
  23. Kim, B-G., Lee, J-H., Yasuda, J., Ryoo, H-M., and Cho, J-Y.: Phospho-Smad1 modulation by Nedd4 E3 ligase in BMP/TGF signaling. Journal of Bone and Mineral Research, 26 (7), 1411-24, 2011.
  24. Fukuma, A., Abe, M., Morikawa, Y., Miyazawa, T., and *Yasuda, J.: Cloning and Characterization of the Antiviral Activity of Feline Tetherin/BST-2. PLoS One, 6, e18247 (1-6), 2011.
  25. Nakaya, Y., Hoshino, S., Yasuda, J., and Miyazawa, T.: Mapping of a neutralizing epitope in the surface envelope protein of porcine endogenous retrovirus subgroup B. Journal of General Virology,92, 940-944, 2011.
  26. Fujinami, Y., Kikkawa, H.S., Kurosaki, Y., Sakurada, K., Yoshino, M., and Yasuda, J.: Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Microbiol Res., 166, 77-86, 2011.
  27. Popkin, DL., Teijaro. JR., Sullivan, BM., Urata, S., Rutschmann, S., de la Torre, JC., Kunz, S., Beutler, B., Oldstone, M.: Hypomorphic mutation in the site-1 protease mbtps1 endows resistance to persistent viral infection in a cell-specific manner. Cell Host Microbe, 9 (3), 212-22, 2011
  28. Nakaya,Y., Shojima, T., Yasuda, J., Imakawa, K., and Miyazawa, T.: Epigenetic regulation on the 5'-proximal CpG island of human porcine endogenous retrovirus subgroup A receptor 2/GPR172B. Microbes Infect., 13, 49-57, 2011.
  29. Fukuma, A., Kurosaki, Y., Morikawa, Y., Grolla, A., Feldmann, H., and *Yasuda, J.: Rapid detection of Lassa virus by reverse transcription – loop-mediated isothermal amplification. Microbiology and Immunology, 55, 44-50, 2011.
  30. Urata, S., Yun, N., Pasquato, A., Paessler, S., Kunz, S., de la Torre, JC.: Antiviral activity of a small-molecule inhibitor of arenavirus glycoprotein processing by the cellular site 1 protease. Journal of Virology, 85 (2), 795-803, 2011
  31. Kurosaki, Y., Grolla, A., Fukuma, A., Feldmann, H., and *Yasuda, J.: Development and evaluation of the simple diagnostic assay for Marburgvirus using reverse transcription-loop-mediated isothermal amplification method. Journal of Clinical Microbiology, 48, 2330-2336, 2010.
  32. Nakaya,Y., Shojima, T., Yasuda, J., and Miyazawa, T.: Unusual permeability of porcine endogenous retrovirus subgroup A through membrane filters. J. Vet. Med. Sci., 72, 67-71, 2010.
  33. Urata, S., and *Yasuda, J.: Regulation of Marburg virus budding by Nedd4.1; a different WW domain of Nedd4.1 is critical for the binding to Marburg and Ebola virus VP40. Journal of General Virology, 91, 228-234, 2010.
  34. Urata, S., Yasuda, J., and de la Torre, J.C.: The Z protein of the New world arenavirus Tacaribe virus has bona fide budding activity that does not depend on known late domain motifs. Journal of Virology, 83 (23), 12651-12655, 2009.
  35. Sakuma, T., Sakurai, A., and *Yasuda, J.: Dimerization of tetherin is not essential for its antiviral activity against Lassa and Marburg viruses. PLoS One, 4, e6934, 2009.
  36. Kurosaki, Y., Sakuma, T., Fukuma, A., Fujinami, Y., Kamo, N., Makino, S., and *Yasuda, J.: A Simple and Sensitive Method for Detection of Bacillus anthracis by Loop-Mediated Isothermal Amplification. Journal of Applied Microbiology, 107, 1947-1956, 2009.
  37. Sakuma, T., Kurosaki, Y., Fujinami, Y., Takizawa, T., and *Yasuda, J.: Rapid and simple detection of Clostridium botulinum types A and B by loop-mediated isothermal amplification. Journal of Applied Microbiology, 106, 1252-1259, 2009.
  38. Sakuma, T., Noda, T., Urata, S., Kawaoka, Y., and *Yasuda, J.: Inhibition of Lassa and Marburg virus production by Tetherin. Journal of Virology, 83 (5), 2382-2385, 2009.
  39. Kikkawa, S. H., Ueda, T., Suzuki, S., and *Yasuda, J.: Characterization of the catalytic activity of the γ-phage lysin, PlyG, specific for Bacillus anthracis. FEMS Microbiology Letters, 286, 236-240, 2008.
  40. Kikkawa, H., Fujinami, Y., Suzuki, S., and *Yasuda, J.: Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of γ-phage, PlyG, to Bacillus anthracis. B.B.R.C., 363, 531-535, 2007.
  41. Urata, S., Yokosawa, H., and *Yasuda, J.: Regulation of HTLV-1 Gag budding by Vps4A, Vps4B, and AIP1/Alix. Virology Journal, 4, 66 (1-5), 2007.
  42. Urata, S., Noda, T., Kawaoka, Y., Morikawa, S., Yokosawa, H., and *Yasuda, J.: Interaction of Tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and Tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP. Journal of Virology, 81 (9), 4895-4899, 2007.
  43. Kurosaki, Y., Takada, A., Ebihara, H., Grolla, A., Kamo, N., Feldmann, H., Kawaoka, Y., and *Yasuda, J.: Rapid and Simple Detection of Ebola Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification. Journal of Virological Methods, 141, 78-83, 2007.
  44. Fujinami, Y., Hirai, Y., Sakai, I., Yoshino, M., and *Yasuda, J.: Sensitive detection of Bacillus anthracis using a binding protein originating from γ-phage. Microbiology and Immunology, 51, 163-169, 2007.
  45. Tsurutani, N., Yasuda, J., Yamamoto, N., Choi, B-I., Kadoki, M., and Iwakura, Y.: Nuclear Import of the Pre-integration Complex Is Blocked upon Infection by HIV-1 in Mouse Cells. Journal of Virology, 81, 677-688, 2007.
  46. Horii, M., Shibata, H., Kobayashi, R., Katoh, K., Yorikawa, C., Yasuda, J., and Maki, M.: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway. Biochemical Journal, 400, 23-32, 2006.
  47. Urata, S., Noda, T., Kawaoka, Y., Yokosawa, H., and *Yasuda, J.: Cellular factors required for Lassa virus budding. Journal of Virology, 80 (8), 4191-4195, 2006.
  48. Katoh, K., Suzuki, H., Terasawa, Y., Mizuno, T., Yasuda, J., Shibata, H., and Maki, M. The penta-EF-hand protein ALG-2 directly interacts with the ESCRT-I component TSG101 and Ca2+-dependently colocalizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B. Biochemical Journal, 391, 677-85, 2005, 2005.
  49. Sakurai, A., Yasuda, J., Takano, H., Tanaka, Y., Hatakeyama, M., and Shida, H.: Regulation of human T-cell leukemia virus type-1 (HTLV-1) budding by ubiquitin ligase Nedd4. Microbes and Infection, 6, 150-156, 2004.
  50. *Yasuda, J., Nakao, M., Kawaoka, Y., and Shida, H.: Nedd4 regulates egress of Ebola virus from host cells. Journal of Virology, 77, 9987-9992, 2003.
  51. Tanaka, J., Ishida, T., Choi, B.-I., Yasuda, J., Watanabe, T., and Iwakura, Y.: Latent HIV-1 reactivation in transgenic mice requires cell cycle-dependent demethylation of CREB/ATF sites in the LTR. AIDS, 17, 167-175, 2003.
  52. Yasuda, J., Hunter, E., Nakao, M., and Shida, H.: Functional involvement of a novel Nedd4-like ubiquitin ligase on retrovirus budding. EMBO Reports, 3, 636-640, 2002.
  53. Yokoyama, H., Yasuda, J., Okamoto, H., and Iwakura, Y.: Development of nephritic syndrome with severe ascites in mice transgenic for the TT virus ORF1 gene. Journal of General Virology, 83, 141-150, 2002.
  54. Yasuda, J., Miyao, T., Kamata, M., Aida, Y., and Iwakura, Y.: T cell apoptosis causes peripheral T cell depletion in mice transgenic for the HIV-1 vpr gene. Virology, 285, 181-192, 2001.
  55. Tanaka, J., Ozaki, H., Yasuda, J., Horai, R., Tagawa, Y., Asano, M., Imai, M., Sekikawa, K., Kopf, M., and Iwakura, Y.: LPS-induced HIV-1 expression in transgenic mice is mediated by TNF-a and IL-1, but not by IFN-g nor IL-6. AIDS, 14, 1299-1307, 2000.
  56. Yasuda, J., and Hunter, E.: Role of Matrix protein in the type-D retrovirus replication cycle: Importance of the arginine residue at position 55. Virology, 268, 533-538, 2000.
  57. Ito, T., Kawaoka, Y., Kameda, C., Yasuda, J., Kida, H., and Otsuki,K.: Differences in receptor specificity between Newcastle disease viruses originating from chickens and waterfowl. J. Vet. Med. Sci., 61, 951-953, 1999.
  58. Ito, T., Kawaoka, Y., Ohira, M., Takakuwa, H., Yasuda, J., Kida, H., and Otsuki, K.: Replacement of internal protein genes, with the exception of the matrix, in equine 1 viruses by equine 2 influenza virus genes during evolution in nature. J. Vet. Med. Sci., 61, 987-989, 1999.
  59. Yasuda, J., and Hunter, E.: A proline-rich motif (PPPY) in the Gag polyprotein of Mason-Pfizer monkey virus plays a maturation-independent role in virion release. Journal of Virology, 72, 4095-4103, 1998.
  60. Yasuda, J., Bucher, D., and Ishihama, A.: Growth control of influenza A virus by M1 protein: Analysis of transfectant viruses carrying the chimeric M gene. Journal of Virology, 68, 8141-8146, 1994.
  61. Kida, H., Ito, T., Yasuda, J., Shimizu, Y., Itakura, C., Shortridge, K.F., Kawaoka, Y., and Webster, R.G.: Potential for transmission of avian influenza viruses to pigs. Journal of General Virology, 75, 2183-2188, 1994.
  62. Yasuda, J., Nakada, S., Kato, A., Toyoda, T., and Ishihama, A.: Molecular assembly of influenza virus: Association of the NS2 protein with virion matrix. Virology;, 196, 249-255, 1993.
  63. Yasuda, J., Toyoda, T., Nakayama, M., and Ishihama, A.: Regulatory effects of matrix protein variations on influenza virus growth. Archives of Virology, 133, 283-294, 1993.
  64. Yasuda, J., Shortridge, K.F., Shimizu, Y., and Kida, H.: Molecular evidence for a role of domestic ducks in the introduction of avian H3 influenza viruses to pigs in southern China, where the A/Hong Kong/68 (H3N2) strain emerged. Journal of General Virology 72, 2007-2010, 1991.

総説・著書 その他

  1. Urata, S., Yasuda, J.: Molecular Mechanism of Arenavirus Assembly and Budding. Viruses, 4(10), 2049-2079, 2012
  2. Yasuda, J.: Ebolavirus Replication and Tetherin/BST-2. Front Microbiol., 3: 111, 2012
  3. Emonet, SE.*, Urata, S.*, de la Torre, JC.*, Arenavirus reverse genetics: New approaches for the investigation of arenavirus biology and development of antiviral strategies. (* Equal contribution), Virology, 411 (2), 416-25, 2011
  4. Urata, S., de la Torre, S.: Arenavirus budding. Advances in Virology, Article ID 180326, 2011
  5. Kurosaki, Y., and *Yasuda, J.: Ebola Viruses. In: Molecular detection of human viral pathogens (Liu, D., ed.), Taylor & Francis CRC Press, 559-572, 2010.
  6. *Yasuda, J.: Marburg virus budding: ESCRT of progeny virion to the outside of the cell. Future Virology, 5, 627-637, 2010.
  7. *Yasuda, J.: Laboratory diagnosis of SARS. In: SARS (Mizutani, T., ed.), Transworld Research Network, 77-87, 2006.
  8. Ishihama, A., Toyoda, T., Kobayashi, M., Yasuda, J., Asano, Y., and Nakayama, M.: Formation and control of influenza virus RNA polymerase. In: Options for the Control of Influenza II (Hannoun, C. et al., eds.), 289-297, 1993.
  9. 安田二朗:ウイルス出芽とESCRT~新生ウイルスを細胞外へエスコートする機構~、"メンブレントラフィックの奔流—分子から細胞、そして個体へ" 蛋白質・核酸・酵素、12月号増刊 53(16)(大野博司、吉森保編)、pp2251-2256、共立出版、東京、2008年。
  10. 安田二朗:全自動モバイル型生物剤センシングシステム、未来材料、11月号、pp58-62、エヌティーエス社、東京、2008年。
  11. 安田二朗:ユビキチンとウイルス出芽、"ユビキチン研究の新展開—メカニズムから疾患研究へ" 別冊•医学のあゆみ(田中啓二編)、pp114-118、医歯薬出版、東京、2006年。
  12. 安田二朗:HIV出芽とTsg101、ウイルス 55(2)、pp281-286、日本ウイルス学会、東京、2005年。
  13. 安田二朗、藤浪良仁:病原微生物の検査、"捜査のための法科学"(高取健彦編)、pp149-160、令文社、東京、2004年。
  14. 安田二朗:ユビキチンとウイルス出芽—ウイルス出芽とMVB sorting pathway、医学のあゆみ 211(1)、pp114-118、医歯薬出版、東京、2004年。
  15. 安田二朗、中尾光善:Nedd4ユビキチンリガーゼファミリーとウイルス出芽機構、現代医療 36(4)、pp69-74、現代医療社、東京、2004年。
  16. 安田二朗、岩倉洋一郎:トランスジェニックマウスの作製法、"実験医学別冊新遺伝子工学ハンドブック"改訂第4版(村松正實、山本雅編)、pp248-253、羊土社、東京、2003年。
  17. 安田二朗、Eric Hunter、中尾光善、志田壽利:ユビキチンリガーゼNedd4樣タンパク質によるレトロウイルスの出芽制御、細胞工学21(10)、pp1222-1223、秀潤社、東京、2002年。
  18. 安田二朗、岩倉洋一郎:トランスジェニックマウスの作製法、"実験医学別冊新遺伝子工学ハンドブック"改訂第3版(村松正實、山本雅編)、pp234-238、羊土社、東京、1999年。