研究内容（電子顕微鏡による研究支援）
微生物や細胞、組織などの微細構造解析を目的として、電子顕微鏡を用いた研究支援を行っています。電子顕微鏡観察のための試料を調整し、電子顕微鏡による観察や画像データを取得します。
主な内容として、ウイルスや細菌、原虫やこれら微生物の感染細胞の微細構造解析や免疫電顕法による分子局在解析を行っています。
研究業績
Hidayati AR, Melinda, Ilmi H, Sakura T, Sakaguchi M, Ohmori J, Hartuti ED, Tumewu L, Inaoka DK, Tanjung M, Yoshida E, Tokumasu F, Kita K, Mori M, Dobashi K, Nozaki T, Syafruddin D, Hafid AF, Waluyo D, Widyawaruyanti A. Effect of geranylated dihydrochalcone from Artocarpus altilis leaves extract on Plasmodium falciparum ultrastructural changes and mitochondrial malate: Quinone oxidoreductase. International Journal for Parasitology: Drugs and Drug Resistance 21: 40-50 (2023).
Furuyama W, Sakaguchi M, Yamada K, Nanbo A. Development of an imaging system for visualization of Ebola virus glycoprotein throughout the viral lifecycle. Frontiers in Microbiology 13: 1026644 (2022).
Chuang H, Sakaguchi M, Lucky BA, Yamagishi J, Katakai Y, Kawai S, Kaneko O. SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells. Scientific Reports 12: 14942 (2022).
Hiyoshi H, English BC, Diaz-Ochoa VE, Wangdi T, Zhang LF, Sakaguchi M, Haneda T, Tsolis RM, Bäumler AJ. Virulence factors perforate the pathogen-containing vacuole to signal efferocytosis. Cell Host & Microbe 30: 163-170 (2022).
Mi-ichi F, Sakaguchi M, Hamano S, Yoshida H. Entamoeba chitinase is required for mature round cyst formation. Microbiology Spectrum 9: e0051121 (2021).
Hakimi H, Templeton TJ, Sakaguchi M, Yamagishi J, Miyazaki S, Yahata K, Uchihashi T, Kawazu SI, Kaneko O, Asada M. Novel Babesia bovis exported proteins that modify properties of infected red blood cells. PLOS Pathogens 16: e1008917 (2020).
Mousa EAA, Sakaguchi M, Nakamura R, Abdella OH, Yoshida H, Hamano S, Mi-ichi F. The dynamics of ultrastructural changes during Entamoeba invadens encystation. Parasitology 147: 1305-1312 (2020).
Zadeh VR, Urata S, Sakaguchi M, Yasuda J. Human BST-2/Tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein. Journal of General Virology 101: 573-586 (2020).
Morimoto Y, Yoshida S, Kinoshita A, Satoh C, Mishima H, Yamaguchi N, Matsuda K, Sakaguchi M, Tanaka T, Komohara Y, Imamura A, Ozawa H, Nakashima M, Kurotaki N, Kishino T, Yoshiura K, Ono S. Nonsense mutation in CFAP43 causes normal-pressure hydrocephalus with ciliary abnormalities. Neurology 92: e2364-e2374 (2019).
Kawai Y, Kawabata C, Sakaguchi M, Tamura T. Protection of baculovirus vectors expressing complement regulatory proteins against serum complement attack. Biological and Pharmaceutical Bulletin 41: 1600-1605 (2018).
Asare KK, SakaguchiM, Lucky BA, AsadaM, Miyazaki S, Katakai Y, KawaiS, SongC, MurataK, YahataK, Kaneko O. The Plasmodium knowlesi MAHRP2 ortholog localizes to structures connecting Sinton Mulligan’s clefts in the infected erythrocyte. Parasitology International 67: 481-492 (2018).
Kyaw Kyaw A, Tun MMN, Buerano CC, Nabeshima T, Sakaguchi M, Ando T, Inoue S, Mya YY, Hayasaka D, Thu HM, Thant KZ, Morita K. Isolation and genomic characterization of Culex flaviviruses from mosquitoes in Myanmar. Virus Research 247: 120-124 (2018).
Iyori M, Yamamoto DS, Sakaguchi M, Mizutani M, Ogata S, Nishiura H, Tamura T, Matsuoka H, Yoshida S. DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice. Malaria Journal 16: 390 (2017).
Kodama Y, Kuramoto H, Mieda Y, Muro T, Nakagawa H, Kurosaki T, Sakaguchi M, Nakamura T, Kitahara T, Sasaki H. Application of biodegradable dendrigraft poly-L-lysine to a small interfering RNA delivery system. Journal of Drug Targeting 25: 49-57 (2017).
Lucky BA, Sakaguchi M, Katakai Y, Kawai S, Yahata K, Templeton TJ, Kaneko O. Plasmodium knowlesi Skeleton-Binding Protein 1 localizes to the ‘Sinton and Mulligan’ stipplings in the cytoplasm of monkey and human erythrocytes. PLoS ONE 11: e0164272 (2016).
Ebine K, Hirai M, Sakaguchi M, Yahata K, Kaneko O, Saito-Nakano Y. Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2. Malaria Journal 15: 323 (2016).
Tamura T, Kawabata C, Matsushita S, Sakaguchi M, Yoshida S. Malaria sporozoite protein expression enhances baculovirus-mediated gene transfer to hepatocytes. Journal of Gene Medicine 18: 75-85 (2016).
Shimada S, Aoki K, Nabeshima T, Fuxun Y, Kurosaki Y, Shiogama K, Onouchi T, Sakaguchi M, Fuchigami T, Ono H, Nishi K, Posadas-Herrera G, Uchida L, Takamatsu Y, Yasuda J, Tsutsumi Y, Fujita H, Morita K, Hayasaka D. Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. Scientific Reports 6: 20213 (2016).
Sakaguchi M, Miyazaki N, Fujioka H, Kaneko O, Murata K. Three-dimensional analysis of morphological changes in the malaria parasite infected red blood cell by serial block-face scanning electron microscopy. Journal of Structural Biology 193: 162-171 (2016).
Mutungi JK, Yahata K, Sakaguchi M, Kaneko O. Isolation of invasive Plasmodium yoelii merozoites with a long half-life to evaluate invasion dynamics and potential invasion inhibitors. Molecular and Biochemical Parasitology 204: 26-33 (2015).
Mutungi JK, Yahata K, Sakaguchi M, Kaneko O. Expression and localisation of rhoptry neck protein 5 in merozoites and sporozoites of Plasmodium yoelii. Parasitology International 63: 794-801 (2014).
Nabeshima T, Inoue S, Okamoto K, Posadas-Herrera G, Yu F, Uchida L, Ichinose A, Sakaguchi M, Sunahara T, Buerano CC, Tadena FP, Orbita IB, Natividad FF, Morita K. Tanay virus, a new species of virus isolated from mosquitoes in the Philippines. Journal of General Virology 95: 1390-1395 (2014).