長崎大学 熱帯医学研究所 病原体解析部門 原虫学分野 麻田

 

  麻田 正仁, DVM, PhD
 

助教
東京大学 農学部 獣医学専修卒業, 2006.
東京大学 大学院農学生命科学研究科 博士課程修了, 2010.
帯広畜産大学 原虫病研究センター 研究機関研究員, 2010-2012.
長崎大学熱帯医学研究所原虫学分野 助教, 2012-現在.

住所   郵便番号852‐8523
長崎市坂本1丁目12‐4
電話/FAX (095)-819-7838 / (095)-819-7805
電子メール    
学術的興味 マラリア、バベシア
手技手法   細胞生物学・分子生物学
研究テーマ :: 業績

Research Map

 
研究テーマ

遺伝子改変弱毒バベシア原虫の開発、マラリア原虫の細胞侵入機構、偶蹄類マラリア
 

業績

原著論文

Kandel RC, Shrestha M, Sadaula A, Khatri-Chhetri M, Maharjan J, Solanki GS, Chalise MK, Asada M, Kaneko O, Poudel RC, Pandey K. First report of the malaria parasite in water buffalo in Nepal. Vet Parasitol (in press)

Rosa C, Asada M, Hakimi H, Domingos A, Pimentel M, Antunes S. Transient transfection of Babesia ovis using heterologous promoters. Ticks Tick Borne Dis 10(6):101279 (2019/10). [PubMed]

Nguyen TT, Dang-Trinh MA, Higuchi L, Mosqueda J, Hakimi H, Asada M, Yamagishi J, Umemiya-Shirafuji R, Kawazu SI. Initiated Babesia ovata Sexual Stages under In Vitro Conditions Were Recognized by Anti-CCp2 Antibodies, Showing Changes in the DNA Content by Imaging Flow Cytometry. Pathogens 8(3): pii: E104 (2019/07). [PubMed]

Hakimi H, Sarani A, Takeda M, Kaneko O, Asada M. Epidemiology, risk factors, and co-infection of vector-borne pathogens in goats from Sistan and Baluchestan province, Iran. PLOS ONE 14(6):e0218609 (2019/06) [PubMed]

Hakimi H, Ishizaki T, Kegawa Y, Kaneko O, Kawazu S, Asada M. Genome editing of Babesia bovis using the CRISPR/Cas9 system. mSphere 4(3):e00109-19 (2019/06) [PubMed]

Kegawa Y, Asada M, Ishizaki T, Yahata K, Kaneko O. Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion. Parasito Int 67(6):706-714 (2018/12) [PubMed] [Open Access]

Asada M, Hakimi H, Kawazu SI. The application of the HyPer fluorescent sensor in the real-time detection of H2O2 in Babesia bovis merozoites in vitro. Vet Parasitol 255:78-82 (2018/05) [PubMed]

Liu M, Adjou Moumouni PF, Asada M, Hakimi H, Masatani T, Vudriko P, Lee SH, Kawazu SI, Yamagishi J, Xuan X. Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni. Parasit Vectors 11(1):260 (2018/04) [PubMed]

Asare KK, Sakaguchi M, Lucky AB, Asada M, Miyazaki S, Katakai Y, Kawai S, Song C, Murata K, Yahata K, Kaneko O. The Plasmodium knowlesi MAHRP2 ortholog localizes to structures connecting Sinton Mulligan's clefts in the infected erythrocyte. Parasitol Int 67:481-492 (2018/04) [PubMed] [Open Access]

Kaewthamasorn M, Takeda M, Saiwichai T, Gitaka JN, Tiawsirisup S, Imasato Y, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi DK, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim AM, Suganuma K, Hakimi H, Nakao R, Katakura K, Asada M, Kaneko O. Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host. Sci Rep 8:5827 (2018/04) [PubMed] [Open Access]

Asada M, Takeda M, Tomas WM, Pellegrin A, de Oliveira CHS, Barbosa JD, da Silveira JAG, Braga EM, Kaneko O. Close relationship of Plasmodium sequences detected from South American pampas deer (Ozotoceros bezoarticus) to Plasmodium spp. in North American white-tailed deer. Int J Parasitol Parasites Wildl 7(1):44-7 (2018/04) [PubMed] [Science Direct Abstract]


Liu M, Adjou Moumouni PF, Cao S, Asada M, Wang G, Gao Y, Guo H, Li J, Vudriko P, Efstratiou A, Ringo AE, Lee SH, Hakimi H, Masatani T, Sunaga F, Kawazu SI, Yamagishi J, Jia L, Inoue N, Xuan X. Identification and characterization of interchangeable cross-species functional promoters between Babesia gibsoni and Babesia bovis. Ticks Tick Borne Dis. 9(2):330-333. (2018/02) [Abstract]

Yamagishi J, Asada M, Hakimi H, Tanaka TQ, Sugimoto C, Kawazu SI. Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens. BMC Genomics. 18(1):832. (2017/10) [Abstract]

Liu M, Asada M, Cao S, Moumouni PFA, Vudriko P, Efstratiou A, Hakimi H, Masatani T, Sunaga F, Kawazu SI, Yamagishi J, Xuan X. Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter. Mol Biochem Parasitol. 216:56-59. (2017/09) [Abstract]

Masatani T, Hayashi K, Andoh M, Tateno M, Endo Y, Asada M, Kusakisako K, Tanaka T, Gokuden M, Hozumi N, Nakadohzono F, Matsuo T. Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan. Ticks Tick Borne Dis. 8(4):581-587. (2017/06) [Abstract]

Musinguzi SP, Suganuma K, Asada M, Laohasinnarong D, Sivakumar T, Yokoyama N, Namangala B, Sugimoto C, Suzuki Y, Xuan X, Inoue N. A PCR-based survey of animal African trypanosomosis and selected piroplasm parasites of cattle and goats in Zambia. J Vet Med Sci 78(12):1819-24 (2017/01) [Abstract]

Masatani T, Asada M, Hakimi H, Hayashi K, Yamagishi J, Kawazu SI, Xuan X. Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis. Parasitol Res 115(8): 3139-3145 (2016/08) [Abstract]

Yamasaki S, Suganuma K, Yamagishi J, Asada M, Yokoyama N, Kawazu S, Inoue N. Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense. Parasit Vectors 9(1):299 (2016/05) [Abstract]

Hakimi H, Yamagishi J, Kegawa Y, Kaneko O, Kawazu SI, Asada M. Establishment of transient and stable transfection systems for Babesia ovata. Parasit Vectors 9:171 (2016/03) [Abstract]

Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O. Ungulate malaria parasites. Sci Rep 6:23230 (2016/03) [Abstract] Access the recommendation on F1000Prime

Laohasinnarong D, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B. Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia. Parasit Vectors 8:497 (2015/09). [Abstract]

Asada M, Yahata K, Hakimi H, Yokoyama N, Igarashi I, Kaneko O, Suarez CE, Kawazu SI. Transfection of Babesia bovis by double selection with WR99210 and Blasticidin-S and its application for functional analysis of thioredoxin peroxidase-1.PLOS ONE 10(5):e0125993 (2015/05) [Abstract]

Mossaad E, Asada M, Nakatani D, Inoue N, Yokoyama N, Kaneko O, Kawazu SI. Calcium ions are involved in egress of Babesia bovis merozoites from bovine erythrocytes. J Vet Med Sci 77(1):53-8 (2015/01) [Abstract]

Hakimi H, Suganuma K, Usui M,Masuda-Suganuma H, Angeles JM, Asada M, Kawai S, Inoue N, Kawazu S. Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity. Parasitol Res 113:3957-3962 (2014/08) [Abstract]

Hakimi H, Angeles Jose Ma, Asada M, Kawai S, Inoue N, Kawazu S. Plasmodium vivax and P. knowlesi: cloning, expression and functional analysis of 1-Cys Peroxiredoxin Exp Parasitol 133(1):101-5 (2013/01) [Abstract]

Suganuma K, Mochabo KM, Hakimi H, Yamasaki S, Yamagishi J, Asada M, Kawazu S, Inoue N. Adenosine-uridine-rich element is one of the required cis-elements for epimastigote form stage-specific gene expression of the congolense epimastigote specific protein. Mol Biochem Parasitol 191(1):36-43 (2013/09) [Abstract]

Suganuma K, Yamasaki S, Asada M, Kawazu S, Inoue N. The epimastigote stage-specific gene expression of CESP is tightly regulated by its 3′ UTR. Mol Biochem Parasitol 186(1):77-80 (2012/11) [Abstract]

Yoshinari T, Sivakumar T, Asada M, Battsetseg B, Huang X, Lan DT, Inpankaew T, Ybanez AP, Alhassan A, Thekisoe OM, Macedo AC, Inokuma H, Igarashi I, Yokoyama N. A PCR Based survey of Babesia ovata in cattle from various Asian, African, and South American countries. J Vet Med Sci 75(2):211-4 (2013/02) [Abstract]

Angeles JM, Goto Y, Kirinoki M, Asada M, Leonardo LR, Rivera PT, Villacorte EA, Inoue N, Chigusa Y, Kawazu S. Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for diagnosis of Schistosoma japonicum Infection among Water Buffaloes. PLoS Negl Trop Dis. 2012 ;6(8):e1800.

Asada M, Goto Y, Yahata K, Yokoyama N, Kawai S, Inoue N, Kaneko O, Kawazu S. Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. PLoS One. 2012;7(4):e35227.

Hakimi H, Asada M, Angeles JM, Inoue N, Kawazu S. Cloning and characterization of Plasmodium vivax thioredoxin peroxidase-1. Parasitol Res. 2012 ;111(2):525-9.

Asada M, Tanaka M, Goto Y, Yokoyama N, Inoue N, Kawazu S. Stable expression of green fluorescent protein and targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis with the WR99210/dhfr selection system. Mol Biochem Parasitol. 2012 ;181(2):162-70.

Goto Y, Duthie MS, Nguyen TT, Asada M, Kawazu S, Carter D, Inoue N. Serological characterizations of tandem repeat proteins for detection of African trypanosome infection in cattle. Parasitol Int. 2011 ;60(4):538-40.

Sanjoba C, Ozbel Y, Asada M, Osada Y, Gantuya S, Matsumoto Y. Recent collections of Sergentomyia squamirostris (Newstead) (Diptera: Psychodidae) in Japan, with descriptions and illustrations. Med Entomol Zool 2011,62:71-77

Ozbel Y, Sanjoba C, Alten B, Asada M, Depaquit J, Matsumoto Y, Demir S, Siyambalagoda RR, Rajapakse RP, Matsumoto Y. Distribution and ecological aspects of sand fly (Diptera: Psychodidae) species in Sri Lanka. J Vector Ecol. 2011 ;36 Suppl 1:S77-86.

Goto Y, Sanjoba C, Asada M, Saeki K, Onodera T, Matsumoto Y. Adhesion of MRP8/14 to amastigotes in skin lesions of Leishmania major-infected mice. Exp Parasitol. 2008 ;119(1):80-6.

著書・その他

Matsumoto Y, Sanjoba C, Asada M, Matsumoto Y. Polymorphism of Leishmaniasis caused by Leishmania donovani s.l. in Asia. Kala Azar in South Asia? current status and challenges ahead (ed. By E. Noiri and T. K. Jha) Springer verlag, 2010

麻田正仁 モンゴル・ゴビ沙漠でのオオスナネズミにおけるリーシュマニア症の調査. 日本獣医寄生虫学雑誌. 2008 ;7:61-66

賞など

日本寄生虫学会 第24回奨励賞 (2015年3月) (業績名:「バイオイメージングによるBabesia bovisメロゾイト滑走運動の研究」)

第20回分子寄生虫学ワークショップ・ベストプレゼンテーション賞 (2012年8月)

2010年度日本獣医寄生虫学奨励賞 (2011年3月)

 

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