Collection of pathogenic protozoa in NEKKEN BRC
Leishmania donovani
Basic information
- Scientific name
- Leishmania donovani
- Biosafety Level
- 2
- Strains
- DD8, D5/260203-3, D7/270203-1, D10/050603-1, D14/080603-1, T4/120603-1, D19/210803-1, D21/240803-1, D26/290803-1, D29/300803-1, D34/060903-1, D36/070903-2, D38/100903-1, D42/280903-1, D45/300903-2, T6/280803-2, T9/030903-2, T11/250903-1
- Item
-
- Cryopreserved living organism
-
- Stage/Form :
- promastigote
- Shipping method :
- frozen, dry ice included, quadruple packing (UN Category B Shipping System)
- Note :
- approximately 1 × 106 cells/vial
- Growing organism in the culture medium
-
- Stage/Form :
- promastigotes in M199 medium
- Shipping method :
- ambient temperature, triple packing (UN Category B Shipping System)
- Note :
- culture with 10 mL of M199 medium
- Extracted DNA
-
- Solvent :
- 100 μl TE buffer, pH 8.0
- Shipping method :
- frozen
- Concentration :
- O.D. 260 ≧ 1.0
Biosafety classification is based on the appended Table 2 (related to the article 2) of the Ministerial Notification No. 7 from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan in 2004.
