Collection of pathogenic protozoa in NEKKEN BRC
Leishmania amazonensis
Basic information
- Scientific name
- Leishmania amazonensis
- Biosafety Level
- 2
- Strains
- LV78
- Item
-
- Cryopreserved living organism
-
- Stage/Form :
- promastigote
- Shipping method :
- frozen, dry ice included, quadruple packing (UN Category B Shipping System)
- Note :
- approximately 1 × 106 cells/vial
- Growing organism in the culture medium
-
- Stage/Form :
- promastigotes in M199 medium
- Shipping method :
- ambient temperature, triple packing (UN Category B Shipping System)
- Note :
- culture with 10 mL of M199 medium
- Extracted DNA
-
- Solvent :
- 100 μl TE buffer, pH 8.0
- Shipping method :
- frozen
- Concentration :
- O.D. 260 ≧ 1.0
Biosafety classification is based on the appended Table 2 (related to the article 2) of the Ministerial Notification No. 7 from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan in 2004.