国際保健学分野
国際保健学分野








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      長崎大学
      熱帯医学研究所
      国際保健学分野

      〒852-8523
      長崎市坂本1-12-4
      TEL(095)819-7869


メンバー


和田 崇之


Takayuki Wada



キーワード
分子疫学、遺伝的多様性、公衆衛生、ゲノム比較、動物感染症、適応進化

研究テーマ

1. 分子疫学、地域公衆衛生

病原体の遺伝多型およびゲノム比較分析に基づいて、病原体の伝搬経路追跡に関する調査・疫学研究、地域特異性に関する遺伝学的解析を行う。分析対象を分離菌株のみならず、臨床検体、野生動物、古病理標本など幅広く設定し、現代社会における病原体の拡がりと定着に至る経緯を究明する。得られた情報を感染症対策と結びつけ、地域公衆衛生や獣医学領域への還元を模索する。

2. 野生動物、飼育動物における感染症と病原体の適応進化

宿主となる生物が様々な病原体に感染する理由は何か。逆に言うと、病原体はなぜ出現するのか。病原性を生命現象における普遍的なふるまいの一つとして捉え、宿主と病原体の共進化や、適応に伴う変遷(伝搬経路や免疫回避、宿主接着性など)に関する現象をターゲットとした研究を行う。具体的には、白血病ウイルスの宿主(ヒトとサル)の感染経路と感染個体数の変遷、抗酸菌(結核菌群など)の抗原性多様性の分子進化、腸管接着性大腸菌の接着因子の多様性などを対象とする。


略歴

1993
1997

1999
2006
1999-2009
2009-2012
2013-2017
2017-

京都大学理学部 入学
京都大学大学院理学研究科博士課程(前期)入学
(再生医科学研究所 永田和宏研)
同 修了
大阪市立大学大学院医学研究科 博士(医学)取得
大阪市立環境科学研究所 研究員
同 研究主任
長崎大学熱帯医学研究所 国際保健学分野 助教
同 准教授


業績
原著論文

2019

Nakamura S, Azuma M, Sato M, Fujiwara N, Nishino S, Wada T, Yoshida S. Pseudo-outbreak of Mycobacterium chimaera through aerators of hand-washing machines at a hematopoietic stem cell transplantation center. Infect Control Hosp Epidemiol. In Press. 2019.


Chen CC, Chang AM, Wada T, Chen MT, Tu YS. Distribution of Carnivore protoparvovirus 1 in free-living leopard cats (Prionailurus bengalensis chinensis) and its association with domestic carnivores in Taiwan. PLoS ONE 14(9): e0221990. 2019.


Kato Y, Wada T, Yamashita H, Ikeda T, Nishiyama K, Imajoh M. Draft genome sequence of Vibrio harveyi strain GAN1807, isolated from diseased greater amberjack (Seriola dumerili) farmed in Nomi Bay, Japan, in 2018. Microbiol Resour Announc 8(34): e00629-19. 2019.


Yamashita H, Wada T, Kato Y, Ikeda T, Imajoh M. Draft genome sequences of three Flavobacterium psychrophilum strains isolated from diseased ayu (Plecoglossus altivelis altivelis) caught at three sites in the Kagami River in Kochi, Japan. Microbiol Resour Announc 8(34): e00773-19. 2019.


Tsai MA, Wang PC, Yoshida S, Aono A, Mitarai S, Wada T, Chen SC. Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex. J Microbiol Methods 164: 105671. 2019.


Yamashita H, Wada T, Kato Y, Ikeda T, Imajoh M. Draft genome sequences of six Flavobacterium psychrophilum strains isolated from dead juvenile ayu (Plecoglossus altivelis altivelis) near the mouth of the Nahari River, Kochi, Japan. Microbiol Resour Announc 8(29): e00759-19. 2019.


Parvej MS, Nakamura H, Alam MA, Wang L, Zhang S, Emura K, Kage-Nakadai E, Wada T, Hara-Kudo Y, Nishikawa Y. Host range-associated clustering based on multi-locus variable-number tandem-repeat analysis, phylotypes, and virulence genes of atypical enteropathogenic Escherichia coli strains. Appl Environ Microbiol 85(6). pii: e02796-18. 2019.


Seto J, Otani Y, Wada T, Suzuki Y, Ikeda T, Araki K, Mizuta K, Ahiko T. Nosocomial Mycobacterium tuberculosis transmission by brief casual contact identified using comparative genomics. J Hosp Infect 102(1): 116–9. 2019.


Yamamoto K, Takeuchi S, Seto J, Shimouchi A, Komukai J, Hase A, Nakamura H, Umeda K, Hirai Y, Matsumoto K, Ogasawara J, Wada T, Yamamoto T. Longitudinal genotyping surveillance of Mycobacterium tuberculosis in an area with high tuberculosis incidence shows high transmission rate of the modern Beijing subfamily in Japan. Infect Genet Evol 72: 25–30. 2019.


2018

Yoshida S, Suga S, Ishikawa S, Mukai Y, Tsuyuguchi K, Inoue Y, Yamamoto T, Wada T. Mycobacterium caprae infection in a captive Borneo elephant. Emerg Infect Dis 24(10): 1937–40. 2018.


Seto J, Wada T, Suzuki Y, Ikeda T, Araki K, Umetsu Y, Ishikawa H, Mizuta K, Ahiko T. A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics. Intl J Tuberc Lung Dis 22(10): 1239–42. 2018.


Kayanuma H, Ogihara K, Yoshida S, Yamamoto K, Wada T, Yamamoto T, Tsuyuki Y, Madarame H. Disseminated nontuberculous mycobacterial disease in a cat caused by Mycobacterium sp. strain MFM001. Vet Microbiol 220: 90–6. 2018.


Yoshida S, Araki T, Asai T, Tsuyuguchi K, Arikawa K, Iwamoto T, Nakajima C, Suzuki Y, Ohya K, Yanai T, Wada T, Yamamoto T. Phylogenetic uniqueness of Mycobacterium avium subspecies hominissuis isolated from an abnormal pulmonary bovine case. Infect Genet Evol 62: 122–9. 2018.


Ohkoshi Y, Sato T, Wada T, Fukushima Y, Murabayashi H, Takakuwa Y, Nishiyama K, Honda H, Shiraishi T, Kuronuma K, Takahashi H, Nakajima C, Suzuki Y, Yokota SI. Whole genome analysis of a multidrug-resistant Streptococcus pneumoniae isolate from a patient with invasive pneumococcal infection developing disseminated intravascular coagulation. J Infect Chemother S1341-321X(18): 30031-X. 2018.


2017

Wada T, Hijikata M, Maeda S, Hang NTL, Thuong PH, Hoang NP, Hung NV, Keicho N. Complete Genome Sequences of Three Representative Mycobacterium tuberculosis Beijing Family Strains Belonging to Distinct Genotype Clusters in Hanoi, Vietnam, during 2007 to 2009. Genome Announc 5(27): e00510-17. 2017.


Wada T, Hijikata M, Maeda S, Hang NTL, Thuong PH, Hoang NP, Hung NV, Keicho N. Complete genome sequence of a Mycobacterium tuberculosis strain belonging to the East African-Indian family in the Indo-Oceanic lineage, isolated in Hanoi, Vietnam. Genome Announc 5(24): e00509-17. 2017.


Yoshida H, Wada T, Taniyama D, Takahashi T. Draft genome sequence of clinical strain TANI1 of Streptococcus suis serotype 5 isolated from a bacteremia patient in Japan. Genome Announc 5(18): e00260-17. 2017.


Seto J, Wada T, Suzuki Y, Ikeda T, Mizuta K, Mitarai S, Ahiko T. Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates. J Microbiol Methods 139: 12–4. 2017.


Li WT, Chang HW, Pang VF, Wang FI, Liu CH, Chen TY, Guo JC, Wada T, Jeng CR. Mycolactone-producing Mycobacterium marinum infection in captive Hong Kong warty newts and pathological evidence of impaired host immune function. Dis Aquat Organ 123(3): 239–49. 2017.


Seto J, Wada T, Suzuki Y, Ikeda T, Mizuta K, Yamamoto T, Ahiko T. Mycobacterium tuberculosis transmission among elderly persons, Yamagata Prefecture, Japan, 2009–2015. Emerg Infect Dis 23(3): 448–55. 2017.


2016

Yokoyama K, Kubo K, Yoshida S, Wada T, Kawai T, Nishikomori R, Yoshida A. You have full text access to this content Tumor in chest wall caused by Mycobacterium bovis BCG infection: Identification on polymerase chain reaction of formalin-fixed specimen. Pediatr Int 58(4): 317–8. 2016.


2015

Wada T, Maruyama F, Iwamoto T, Maeda S, Yamamoto T, Nakagawa I, Yamamoto S, Ohara N. Deep sequencing analysis of the heterogeneity of seed and commercial lots of the bacillus Calmette-Guérin (BCG) tuberculosis vaccine substrain Tokyo-172. Sci Rep. 5: 17827. 2015.


Ban E, Yoshida Y, Wakushima M, Wajima T, Hamabata T, Ichikawa N, Abe H, Horiguchi Y, Hara-Kudo Y, Kage-Nakadai E, Yamamoto T, Wada T, Nishikawa Y. Characterization of unstable pEntYN10 from enterotoxigenic Escherichia coli (ETEC) O169:H41. Virulence. 6(8): 735–44. 2015.


Seto J, Wada T, Iwamoto T, Tamaru A, Maeda S, Yamamoto K, Hase A, Murakami K, Maeda E, Oishi A, Migita Y, Yamamoto T, Ahiko T. Phylogenetic assignment of Mycobacterium tuberculosis Beijing clinical isolates in Japan by maximum a posteriori estimation. Infect Genet Evol. 35: 82–8. 2015.


Yokoyama E, Hachisu Y, Iwamoto T, Nakanishi N, Arikawa K, Wada T, Seto J, Kishida K. Comparative analysis of Mycobacterium tuberculosis Beijing strains isolated in three remote areas of Japan. Infect Genet Evol 34: 444–9. 2015.


Wada T, Iwamoto T, Tamaru A, Seto J, Ahiko T, Yamamoto K, Hase A, Maeda S, Yamamoto T. Clonality and micro-diversity of a nationwide spreading genotype of Mycobacterium tuberculosis in Japan. PLoS ONE 2015: 10(3): e0118495. 2015.


Teramoto K, Suga M, Sato T, Wada T, Yamamoto A, Fujiwara A. Characterization of mycolic acids in total fatty acid methyl ester fractions from Mycobacterium species by high resolution MALDI-TOFMS. Mass Spectrometry. 4: A0035. 2015.


Collins J, Ichikawa T, Abdu H, Ocheke I, Modise-Letsatsi UV, Wada T, Okolo S, Yamamoto T. Maternal Overweight/Obesity Characteristics and Child Anthropometric Status in Jos, Nigeria. Niger Med J 56(4): 236–9. 2015.


Okano Y, Yoshida S, Shinohara T, Takahashi N, Naito N, Kagawa K, Machida H, Hatakeyama N, Ohno A, Wada T, Ogushi F. Primary gingival tuberculosis diagnosed by genetic identification. Intern Med 54(21): 2765–8. 2015.


Wada T, Iwamoto T, Tamaru A, Seto J, Ahiko T, Yamamoto K, Hase A, Maeda S, Yamamoto T. Clonality and micro-diversity of a nationwide spreading genotype of Mycobacterium tuberculosis in Japan. PLoS ONE 10(3):e0118495. 2015.


2014

Umeda K, Hatakeyama R, Abe T, Takakura K, Wada T, Ogasawara J, Sanada S, Hase A. Distribution of Capnocytophaga canimorsus in dogs and cats with genetic characterization of isolates. Vet Microbiol. 171(1–2): 153–9. 2014.


Haridy M, Tachikawa Y, Yoshida S, Tsuyuguchi K, Tomita M, Maeda S, Wada T, Ibi K, Sakai H, Yanai T. Mycobacterium marinum infection in Japanese forest green tree frogs (Rhacophorus arboreus). J Comp Pathol. 151(2–3): 277–89. 2014.


de Beer JL, Kodmon C, Van Ingen J, Supply P, Van Soolingen D, Jamieson FB, Bidovec-Stojkovic U, Brown T, Cirillo, DM, Cruz L, Miranda A, Dou HY, Fauville-Dufaux M, Fitzgibbon MM, Garcia De Viedma D, Groenheit R, Haanpera-Heikkinen M, Indra A, Kam KM, Kramer R, Jiang GL, Niemann S, Obrovac M, Rasmussen EM, Refregier G, Realpe T, Samper S, Sharma MK, Sougakoff W, Stakenas P, Stavrum R, Trenkler J, Wada T, Siame KK, Tafaj S, Cowan L, Sng LH, Seagar AL, Basu I, Rastogi N, Ferro BE, De Matos, F, Kipnis A, Van Soolingen D, Supply P. Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex. Int J Tuberc Lung Dis 18(5): 594–600. 2014.


2013

Tateishi Y, Tamaru A, Ogura Y, Niki M, Wada T, Yamamoto T, Hirata K, Hayashi T, Matsumoto S. Whole-genome sequence of the potentially hyper-transmissible multi-drug resistant Mycobacterium tuberculosis Beijing strain, OM-V02_005. Genome Announc 1(4): e00608-13. 2013.


Umeda K, Wada T, Kohda T, Kozaki S. Multi-locus Variable number tandem repeat analysis for Clostridium botulinum type B isolates in Japan: comparison with other isolates and genotyping methods. Infect Genet Evol 16: 298–304. 2013.


瀬戸順次, 阿彦忠之, 和田崇之, 長谷篤, 山田敬子. 結核低蔓延地域における網羅的な結核菌反復配列多型(VNTR)分析の有用性.結核 88(6): 535–42. 2013.


Nakanishi N, Wada T, Arikawa K, Millet J, Rastogi N, Iwamoto T. Evolutionary robust SNPs reveal the misclassification of Mycobacterium tuberculosis Beijing family strains into sublineages. Infect Genet Evol 16: 174–7. 2013.


田丸亜貴, 和田崇之, 岩本朋忠, 長谷篤. JATA(12)-VNTR型別による結核集団発生事例の菌株異同調査. 結核 88(4): 399–43. 2013.


和田崇之, 田丸亜貴, 岩本朋忠, 有川健太郎, 中西典子, 小向潤, 松本健二, 長谷篤. 複数自治体をまたぐ広域的結核分子疫学の基盤構築 ―JATA(12)-VNTR型別に基づくクラスター形成とその傾向―. 結核 88(4): 393–8. 2013.


Wada T, Maeda S. Multiplex agarose gel electrophoresis system for variable number of tandem repeats genotyping: analysis example using Mycobacterium tuberculosis. Electrophoresis 34(8):1171–4. 2013.


2012

Iwamoto T, Grandjean L, Arikawa K, Nakanishi N, Caviedes L, Coronel J, Sheen P, Wada T, Taype CA, Shaw MA, Moore DA, Gilman RH. Genetic diversity and transmission characteristics of Beijing family strains of Mycobacterium tuberculosis in Peru. PLoS ONE 7(11): e49651. 2012.


Tamaru A, Nakajima C, Wada T, Wang Y, Inoue M, Kawahara R, Maekura R, Ozeki Y, Ogura H, Kobayashi K, Suzuki Y, Matsumoto S. Dominant incidence of multidrug and extensively drug-resistant specific Mycobacterium tuberculosis clones in Osaka Prefecture, Japan. PLoS ONE 7(8): e42505. 2012.


Wada T, Iwamoto T, Hase A, Maeda S. Scanning of genetic diversity of evolutionarily sequential Mycobacterium tuberculosis Beijing family strains based on genome wide analysis. Infect Genet Evol 12(7): 1392–6. 2012.


de Beer JL, Kremer K, Kodmon C, Supply P, van Soolingen D; Global Network for the Molecular Surveillance of Tuberculosis 2009. First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains. J Clin Microbiol 50(3): 662–9. 2012.


2010

Kang HY, Wada T, Iwamoto T, Maeda S, Murase Y, Kato S, Kim HJ, Park YK. Phylogeographical particularity of the Mycobacterium tuberculosis Beijing family in South Korea based on international comparison among surrounding countries. J Med Microbiol 59(10): 1191–7. 2010.


Maeda S, Wada T, Iwamoto T, Murase Y, Mitarai S, Sugawara I, Kato S. Beijing family Mycobacterium tuberculosis isolated from throughout Japan: phylogeny and genetic features. Int JTubercLung Dis 14(9): 1201–4. 2010.


2009

Iwamoto T, Fujiyama R, Yoshida S, Wada T, Shirai C, Kawakami Y. Population structure dynamics of Mycobacterium tuberculosis Beijing strains during previous decades in Japan. J Clin Microbiol 47(10): 3340–3. 2009


Fujihara S, Arikawa K, Aota T, Tanaka H, Nakamura H, Wada T, Hase A, Nishikawa Y. Prevalence and properties of diarrheagenic Escherichia coli among healthy individuals in Osaka City, Japan. Jpn J Infect Dis 62(4):318–23. 2009.


Wada T, Iwamoto T. Allelic diversity of variable number of tandem repeats provides phylogenetic clues regarding the Mycobacterium tuberculosis Beijing family. Infect Genet Evol 9(5):921–6. 2009.


Wada T, Fujihara S, Shimouchi A, Harada M, Ogura H, Matsumoto S, Hase A. High transmissibility of the modern Beijing Mycobacterium tuberculosis in homeless patients of Japan. Tuberculosis 89(4): 252–5. 2009.


Wada T, Iwamoto T, Maeda S. Genetic diversity of the Mycobacterium tuberculosis Beijing family in East Asia revealed through refined population structure analysis. FEMS Microbiol Lett 291(1): 35–43. 2009.


2008

Iwamoto T, Yoshida S, Suzuki K, Wada T. Population structure analysis of the Mycobacterium tuberculosis Beijing family indicates an association between certain sublineages and multidrug resistance. Antimicrob Agents Chemother. 52(10):3805–9. 2008.


2007

Wada T, Maeda S, Hase A, Kobayashi K. Evaluation of variable numbers of tandem repeat as molecular epidemiological markers of Mycobacterium tuberculosis in Japan. J Med Microbiol 56(8):1052–7. 2007.


2004

Wada T, Maeda S, Tamaru A, Imai S, Hase A, Kobayashi K. Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR. J Clin Microbiol 42(11):5277–85. 2004.


Aoki K, Matsumoto S, Hirayama Y, Wada T, Ozeki Y, Niki M, Domenech P, Umemori K, Yamamoto S, Mineda A, Matsumoto M, Kobayashi K. Extracellular mycobacterial DNA-binding protein 1 participates in mycobacterium-lung epithelial cell interaction through hyaluronic acid. J Biol Chem 279(38): 39798–806. 2004.


2002

Nishikawa Y, Zhou Z, Hase A, Ogasawara J, Kitase T, Abe N, Nakamura H, Wada T, Ishii E, Haruki K; Surveillance Team. Diarrheagenic Escherichia coli isolated from stools of sporadic cases of diarrheal illness in Osaka City, Japan between 1997 and 2000: prevalence of enteroaggregative E. coli heat-stable enterotoxin 1 gene-possessing E. coli. Jpn J Infect Dis 55(6):183–90. 2002.


Sato K, Yomogida K, Wada T, Yorihuzi T, Nishimune Y, Hosokawa N, Nagata K. Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary. J Biol Chem 277(40):37678–84. 2002.


邦文(総説、解説など)

2019

和田崇之. シリーズ「世界の結核研究の動向」:ゲノム科学の奔流と結核分子疫学. 複十字 印刷中. 2019.


2017

和田崇之, 吉田志緒美, 柳井徳磨. ヒト,動物,環境をとりまく非結核性抗酸菌の浸淫状況と宿主適応. 日本臨床微生物学雑誌 27(3): 1–10. 2017.


2015

山本太郎, 和田崇之, 市川智生. 進化とエコヘルス. 医学の歩み 250(3): 230–4. 2015.


和田崇之, 吉田志緒美, 山本太郎. 抗酸菌属に潜む分類学と臨床現場の乖離. 生物の科学「遺伝」 69(5): 415–20. 2015.


2012

和田崇之. 視点~結核分子疫学体制の今:克服すべき課題と展望について. 公衆衛生 76(1): 2–3. 2012.


2011

和田崇之, 前田伸司. ゲノム配列にもとづく結核菌群の発生と進化の理解. 化学療法の領域 27(6): 25–31. 2011.


2010

和田崇之, 長谷篤. 結核菌縦列反復配列 (VNTR) 解析に基づく分子疫学的活用とその展望 –大阪市の例. 結核 85(12): 845–52. 2010.


2009

和田崇之, 前田伸司. これ知っとこう!⑥「北京株」. 保健師・看護師の結核展望 47(1): 86–9. 2009.


2008

前田伸司, 和田崇之. 抗酸菌の分子疫学解析とその応用. Medical Technology 36(2): 170–5. 2008.


和田崇之, 前田伸司. これ知っとこう!⑤「VNTR」. 保健師・看護師の結核展望 46(2): 86–7. 2008.


和田崇之, 前田伸司. 抗酸菌の分子疫学法. 呼吸器科 13(1): 92–8. 2008.


2007

和田崇之. 薬剤耐性結核菌の迅速検出法. 生活衛生 51(3): 141–8. 2007.



競争的資金(代表のみ)

科研費

挑戦的研究(萌芽) (2019-2020)
    「病原細菌の宿主適応とニッチ拡散:定着因子の分子進化から拓く微生物生態学の新展開」

基盤研究B (2017-2019)
    「患者地理情報と病原体遺伝子情報を駆使した結核伝播経路追跡」

基盤研究B 海外学術調査 (2015-2018)
    「アジア・オセアニアにおける病原性抗酸菌の浸淫度調査」

挑戦的萌芽研究 (2014-2015)
    「古標本から紐解く結核史と結核分子疫学へのフィードバック」

若手研究A (2012-2014)
    「牛型結核菌の宿主適応に関する遺伝的特性と多様性解析」

若手研究B (2010-2011)
    「広域的な拡散が懸念される結核菌臨床分離株の疫学情報・ゲノム情報に関する統合的研究」


民間研究助成

かなえ医薬振興財団 アジア・オセアニア交流研究助成 (2017)
    「野生動物から分離される大腸菌の腸管付着因子はいかにして宿主適応しているのか?」

発酵研究所 一般研究助成 [分類・生態・進化] (2010)
    「多様な時系列標本から分離されるヒト結核菌のゲノム情報抽出と分子進化学的検討」

黒住医学研究振興財団 研究助成 [疫学] (2008)
    「ゲノム比較および疫学的情報に基づいた結核菌の統合的理解と分子型別監視体制の確立」

大同生命厚生事業団 科学研究助成 (2007)
    「結核菌の新規分子疫学的手法MIRU-VNTR法の評価および応用」


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Copyright : 2008 Department of International Health, Institute of tropical medicine, Nagasaki university.

E-MAIL : y-taro@nagasaki-u.ac.jp