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Research Article
[Invasion of the erythrocytes]
Kaneko O, Tsuboi T, Ling IT, Howell S, Shirano M, Tachibana M,
Cao YM, Holder AA, Torii M. The high molecular mass rhoptry protein, RhopH1, is encoded by
members of the clag multigene family in Plasmodium
falciparum and Plasmodium yoelii. Mol Biochem Parasitol 118(2):223-31
(2001).
[PubMed]
Mayer DCG, Kaneko O,
Hudson-Taylor D, Reid ME, Miller LH. Characterization of a Plasmodium falciparum
erythrocyte binding protein paralogous to EBA-175.
Proc Natl Acad Sci U. S. A. 98(9):
5222-7 (2001)
[PubMed]
[Full Text]
Shirano M, Tsuboi T, Kaneko O, Tachibana M, Adams
JH, Torii M. Conserved Regions of the Plasmodium yoelii Rhoptry Protein
RhopH3 Revealed by Comparison with the P. falciparum
Homologue. Mol Biochem Parasitol 112: 279-9 (2001)
[PubMed, no abstract]
Kaneko O, Fidock DA, Schwartz OM, Miller LH. Disruption of the C-terminal region of EBA-175 in the Dd2/Nm clone
of Plasmodium falciparum does not affect erythrocyte
invasion. Mol Biochem Parasitol 110(1):
135-146 (2000)
[PubMed]
Kaneko O, Soubes SC, Miller LH, Plasmodium falciparum: Invasion of Aotus monkey
red blood
cells and adaptation to Aotus monkeys. Exp Parasitol 93: 116-119 (1999)
[PubMed, no abstract]
Soubes SC, Reid ME,
Kaneko O, Miller LH. Search for the Sialic Acid-Independent Receptor on Red Blood Cells
for Invasion by Plasmodium falciparum. Vox Sang
76(2): 107-114 (1999)
[PubMed]
[Molecular Epidemiology of malaria parasites]
Kimura M,
Kaneko O, Inoue A, Ishii A, Tanabe K. Amplification by polymerase chain reaction of Plasmodium
falciparum DNA from Giemsa-stained thin blood smears.
Mol Biochem
Parasitol 70: 193-197 (1995)
[PubMed, no abstract]
Kaneko O, Jongwutiwes S, Kimura M, Kanbara H, Ishii A, Tanabe K. Plasmodium falciparum:
variation in block 4 of the precursor to the major merozoite surface
proteins in natural populations.
Exp Parasitol
84: 92-95 (1996)
[PubMed, no abstract]
Kaneko O, Kimura M, Kawamoto F, Ferreira MU, Tanabe K. Plasmodium falciparum:
Allelic variation in the merozoite surface protein 1 gene in wild
isolates from Southern Vietnam.
Exp Parasitol 86: 45-57 (1997)
[PubMed]
Ferreira MU, Liu Q, Zhou M, Kimura M,
Kaneko O, Thien HV, Isomura S, Tanabe K, Kawamoto F. Stable patterns of allelic diversity at the Merozoite surface
protein-1 locus of Plasmodium falciparum in Clinical isolates
from southern Vietnam.
J Eukaryot Microbiol 45(1): 131-136 (1998)
[PubMed]
Ferreira MU, Liu Q, Kaneko O, Kimura M,
Tanabe K, Kimura EAS, Kazin AM, Isomura S, Kawamoto F. Allelic diversity at the merozoite surface protein-1 locus of
Plasmodium falciparum in clinical isolates from the southwestern
Brazilian Amazon.
Am J Trop Med Hyg 59(3): 474-480 (1998)
[PubMed]
Tanabe K, Sakihama N, Nakamura Y, Kaneko O,
Kimura M, Ferreira MU, Hirayama K. Selection and genetic drift of polymorphisms
within the merozoite surface protein-1 gene of Plasmodium
falciparum. Gene
241(2): 325-331 (2000)
[PubMed]
Alexandre JSF, Kaewthamasorn, Yahata K, Nakazawa S, Kaneko O. Positive selection on the Plasmodium falciparum clag2 gene encoding a component of the erythrocyte-binding rhoptry protein complex. Trop Med Health 39(3):77-82 (2011). [PubMed]
Sungkapong T, Culleton R, Yahata K, Tachibana M, Ruengveerayuth R, Udomsangpetch R, Torii M, Tsuboi T, Sattabongkot J, Kaneko O, Chotivanich K. Humoral immune responses to Plasmodium vivax subtelomeric transmembrane proteins in Thailand. Southeast Asian J Trop Med Public Health 42(6):1313-21 (2011/11) [PubMed]
Tang J, Dai Y, Zhang H, Culleton Rl, Liu Y, Zhao S, Wang X, Guan X, Kaneko O, Zhu Y. Positive diversifying selection on Plasmodium vivax RON2 protein. Parasitology 139(6):709-15 (2012/05) [PubMed]
Kaewthamasorn M, Yahata K, Alexandre JSF, Xangsayarath P, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O. Stable allele frequency distribution of the polymorphic region of SURFIN4.2 in Plasmodium falciparum isolates from Thailand. Parasitol Int 61(2):317-23 (2012/06) [PubMed]
Alexandre JSF, Xangsayarath P, Kaewthamasorn, Yahata K, Sattabongkot J, Udomsangpetch R, Kaneko O. Stable allele frequency distribution of the Plasmodium falciparum clag genes encoding components of the high molecular weight rhoptry protein complex. Trop Med Health 40(3):71-7 (2012/09) [PubMed]
Xangsayarath P, Kaewthamasorn K, Yahata K, Nakazawa S, Sattabongkot J, Udomsangpetch R, Kaneko O. Positive diversifying selection on the Plasmodium falciparum surf4.1 gene in Thailand. Trop Med Health 40(3):79-89 (2012/09) [PubMed]
Miao M, Wang Z, Yang Z, Yuan L, Parker DM, Putaporntip C, Jongwutiwes S, Xangsayarath P, Pongvongsa T, Moji H, Dinh Tuong T, Abe T, Nakazawa S, Kyaw MP, Yan G, Sirichaisinthop J, Sattabongkot J, Mu J, Su XZ, Kaneko O, Cui L. Genetic Diversity and Lack of Artemisinin Selection Signature on the Plasmodium falciparum ATP6 in the Greater Mekong Subregion. PLoS ONE 8(3):e59192.(2013) [PubMed]
Osborne A, Manko E, Takeda M, Kaneko A, Kagaya W, Chan C, Ngara M, Kongere J, Kita K, Campino S, Kaneko O, Gitaka J, Clark TG. Characterizing the genomic variation and population dynamics of Plasmodium falciparum malaria parasites in and around Lake Victoria, Kenya. Sci Rep 11:19809. (2021/10) [PubMed] doi: 10.1038/s41598-021-99192-1
Nundu SS, Culleton R, Simpson SV, Arima H, Chitama BYA, Muyembe JJ, Ahuka S, Kaneko O, Mita T, Yamamoto T. Identification of polymorphisms in genes associated with drug resistance in Plasmodium falciparum isolates from school-age children in Kinshasa, Democratic Republic of Congo . Parasitol Int (in press)
[Others articles related to Plasmodium]
Kawamoto F, Miyake H, Kaneko O, Kimura M, Nguyen TD, Nguyen
TD, Liu Q, Zhou M, Le DD, Kawai S, Isomura S, Wataya Y, Sequence variation in the 18S rRNA gene, a target for PCR-based
malaria diagnosis, in Plasmodium ovale from southern Vietnam.
J
Clin Microbiol 34: 2287-2289 (1996)
[PubMed]
[Reprint]
Kimura M, Kaneko O, Liu Q, Zhou M, Kawamoto F, Wataya S, Otani
S, Yamaguchi Y, Tanabe K. Identification of the four species of human malaria parasites by
nested PCR that targets various sequences in the small subunit rRNA
gene. Parasitology
International 46(2): 91-95 (1997)
[PubMed]
Kaneko A, Kaneko O, Taleo G, Bjorkman A, Kobayakawa T. High frequencies of CYP2C19 mutations and poor metabolism of
proguanil in Vanuatu. The Lancet 349:
921-922 (1997)
[PubMed, no abstract]
Kaneko A, Bergqvist Y, Takechi M, Kalkoa M, Kaneko O,
Kobayakawa T, Ishizaki T, Bjorkman A. Intrinsic Efficacy of Proguanil Against Falciparum and Vivax Malaria
Independent of the Metabolite Cycloguanil. J Infect Dis 179(4): 974-979 (1999)
[PubMed]
[Reprint]
Nagao E, Kaneko O, Dvorak JA, Plasmodium falciparum-Infected
Erythrocytes: Qualitative and Quantitative Analyses of Parasite-Induced Knobs by
Atomic Force Microscopy. J Struct Biol
130(1): 34-44 (2000)
[PubMed]
|
Tachibana M, Tsuboi T, Templeton TJ, Kaneko O, Torii M.
Presence of three distinct ookinete surface protein genes, Pos25,
Pos28-1, and Pos28-2 in Plasmodium ovale.
Mol Biochem Parasitol 113:341-4 (2001)
[PubMed, no abstract]
Tachibana M, Tsuboi T, Kaneko O, Khuntirat B, Torii M. Two types of Plasmodium ovale defined by SSU rRNA
have distinct sequences for ookinete surface proteins.
Mol Biochem Parasitol 122:223-6 (2002)
[PubMed, no abstract]
Tsuboi T,
Kaneko O, Eitoku C, Suwanabun N, Sattabongkot J, Vinetz JM, Torii M. Gene structure and ookinete expression of the chitinase genes of
Plasmodium vivax and Plasmodium yoelii.
Mol Biochem Parasitol 130(1):51-4 (2003).
[PubMed, no abstract]
Tsuboi T, Kaneko O, Cao
YM, Tachibana M, Yakushijin Y, Nagao T, Kanbara H, Torii M. A
rapid genotyping method for the vivax malaria transmission-blocking
vaccine candidates, Pvs25 and Pvs28.
Parasitol Int 53:211–6 (2004)
[PubMed]
Kaneko O, Templeton TJ, Iriko H, Tachibana M, Otsuki H, Takeo S,
Sattabongkot J, Torii M, Tsuboi T. The Plasmodium vivax
homolog of the ookinete adhesive micronemal protein, CTRP
Parasitol Int 55:227-231 (2006)
[PubMed]
Tsuboi T, Takeo
S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, Han E-T, Otsuki H, Kaneko O,
Sattabongkot J, Udomsangpetch R, Sawasaki T, Torii M, Endo Y. The wheat germ
cell-free based production of malaria proteins for discovery of novel vaccine
candidates.
Infect Immun 76(4):1702-1708
(2008). [PubMed]
Wang Y, Kaneko O, Sattabongkot J, Chen J-H, Lu F, Chai J-Y, Takeo S, Tsuboi T, Ayala FJ, Chen Y, Lim CS, Han ET. Genetic Polymorphism of Plasmodium vivax msp1p, a Paralog of Merozoite Surface Protein 1, from Worldwide Isolates. Am J Trop Med Hyg 84(2), 292-297 (2011/02).[PubMed]
Tsumori Y, Ndounga M, Sunahara T, Hayashida N, Inoue M, Nakazawa S, Casimiro P, Isozumi R, Uemura H, Tanabe K, Kaneko O, Culleton R. Plasmodium falciparum: Differential selection of drug resistance alleles in contiguous urban and peri-urban areas of Brazzaville, Republic of Congo. PLoS ONE 6(8), e23430 (2011/08).[PubMed]
Tachibana M, Sato C, Otsuki H, Sattabongkot J, Kaneko O, Torii M, Tsuboi T. Plasmodium vivax gametocyte protein Pvs230 is a
transmission-blocking vaccine candidate. Vaccine 30(10):1807-12 (2012/02).[PubMed]
Inoue M, Jianxia T, Miyakoda M, Kaneko O, Yui K, Culleton R. The species specificity of immunity generated by live whole organism immunization with erythrocytic and pre-erythrocytic stages of rodent malaria parasites and implications for vaccine development Int J Parasitol 42(9):859-70 (2012). [PubMed]
Tachibana S, Sullivan SA, Kawai S, Nakamura S, Kim HR, Goto N, Arisue N, Palacpac NMQ, Honma H, Yagi M, Tougan T, Katakai Y, Kaneko O, Mita T, Kita K, Yasutomi Y, Sutton PL, Shakhbatyan R, Horii T, Yasunaga T, Barnwell JW, Escalante AA, Carlton JM, Tanabe K. Plasmodium cynomolgi genome sequences provide insight into Plasmodium vivax and the monkey malaria clade. Nat Genet 44(9):1051-5 (2012). [PubMed]
Morita M, Sanai H, Hiramoto A, Sato A, Hiraoka O, Sakura T, Kaneko O, Masuyama A, Nojima M, Wataya Y, Kim H-S Plasmodium falciparum endoplasmic reticulum-resident calcium binding protein is a possible target of synthetic antimalarial endoperoxides, N-89 and N-251. J Proteome Res 11(12):5704-11 (2012) [PubMed]
Zhao H, Konishi A, Fujita Y, Yagi M, Ohata K, Aoshi T, Itagaki S, Sato S, Narita H, Abdelgelil NH, Inoue M, Culleton R, Kaneko O, Nakagawa A, Horii T, Akira S, Ishii KJ, Coban C. Lipocalin 2 bolsters innate and adaptive immune responses to blood-stage malaria infection by reinforcing host iron metabolism. Cell Host Microbe 12(5), 705-16 (2012/11). [PubMed]
Pattaradilokrat S, Li J, Wu J, Qi Y, Eastman RT, Zilversmit M, Nair SC, Huaman MC, Quinones M, Jiang H, Li N, Zhu J, Zhao K, Kaneko O, Long CA, Su XZ. Plasmodium genetic loci linked to host cytokine and chemokine responses. Genes Immun 15(3):145-52 (2014/04-05) [PubMed]
Abkallo HM, Liu W, Hokama S, Ferreira PE, Nakazawa S, Maeno Y, Quang NT, Kobayashi N, Kaneko O, Huffman MA, Kawai S, Marchand RP, Carter R, Hahn BH, Culleton R. DNA from pre-erythrocytic stage malaria parasites is detectable by PCR in the faeces and blood of hosts. Int J Parasitol 44(7):467-73 (2014/06) [PubMed]
Tachibana M, Suwanabun N, Kaneko O, Iriko H, Otsuki H, Sattabongkot J, Kaneko A, Herrera S, Torii M, Tsuboi T. Plasmodium vivax gametocyte proteins, Pvs48/45 and Pvs47, induce transmission-reducing antibodies by DNA immunization. Vaccine 33(16):1901-8 (2015/04) [PubMed]
Ebine K, Hirai M, Sakaguchi M, Yahata K, Kaneko O, Saito-Nakano Y. Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2. Malar J 15(1):323 (2016/06) [PubMed]
Zhu X, He Y, Liang Y, Kaneko O, Cui L, Cao Y. Tryptophan-rich domains of Plasmodium falciparum SURFIN4.2 and Plasmodium vivax PvSTP2 interact with membrane skeleton of red blood cell. Malar J 16(1):121 (2017/03) [PubMed].
Ishikawa T,Mizuta S, Kaneko O, Yahata K. Fragment molecular orbital study of the interaction between sarco/endoplasmic reticulum Ca2+-ATPase and its inhibitor thapsigargin toward anti-malarial development. J Phys Chem B 122(33):7970-7977 (2018/08) [PubMed]
Muh F, Ahmed MA, Han JH, Nyunt MH, Lee SK, Lau YL, Kaneko O, Han ET. Cross-species analysis of apical asparagine-rich protein of Plasmodium vivax and Plasmodium knowlesi. Sci Rep 8(1):5781 (2018/04) [PubMed]
Mosaddeque F, Mizukami S, Kamel MG, Teklemichael AA, Dat TV, Mizuta S, Toan DV, Ahmed AM, Vuong NL, Elhady MT, Giang HTN, Dang TN, Fukuda M, Huynh LK, Tanaka Y, Egan TJ, Kaneko O, Huy NT, Hirayama K. Prediction model for anti-1 malarial activities of hemozoin inhibitors using physicochemical properties. Antimicrob Agents Chemother 62(5):e02424-17 (2018/04) [PubMed]
Kijogia C, Kimura D, Bao LQ, Nakamura R, Chadeka EA, Cheruiyot NB, Bahati F, Yahata K, Kaneko O, Njenga SM, Ichinose Y, Hamano S, Yui K. Modulation of immune responses by Plasmodium falciparum infection in asymptomatic children living in the endemic region of Mbita, western Kenya. Parasitol Int S1383-5769(17):30518-4 (2018/06) [Abstract]
Hakimi H, Sarani A, Takeda M, Kaneko O, Asada M. Epidemiology, risk factors, and co-infection of vector-borne pathogens in goats from Sistan and Baluchestan province, Iran. PLOS ONE 14(6):e0218609 (2019/06) [PubMed]
Morita M, Hayashi K, Sato A, Hiramoto A, Kaneko O, Isogawa R, Kurosaki Y, Miyoshi S, Chang KS, Wataya Y, Kim HS. Genomic and biological features of Plasmodium falciparum resistance against antimalarial endoperoxide N-89. Gene 716:144016 (2019/10) [PubMed]
Kandel RC, Shrestha M, Sadaula A, Khatri-Chhetri M, Maharjan J, Solanki GS, Chalise MK, Asada M, Kaneko O, Poudel RC, Pandey K. First report of the malaria parasite in water buffalo in Nepal. Vet Parasitol Reg Stud Reports 18:100348 (2019/12) [PubMed] doi: 10.1016/j.vprsr.2019.100348
Kawai S, Annoura T, Araki T, Shiogama Y, Shogo S, Sato MO, Kaneko O, Yasutomi Y, Chigusa Y. Development of an effective alternative model for in vivo hypnozoite-induced relapse infections: a Japanese macaque (Macaca fuscata) model experimentally infected with Plasmodium cynomolgi. Parasitol Int 76:102096 (2020/06) [PubMed] doi: 10.1016/j.parint.2020.102096
Muh F, Nyunt MH, Han JH, Firdaus ER, Hoque MR, Lee SK, Kim N, Park JH, Moon RW, Lau YL, Kaneko O, Han ET. Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to P. knowlesi. PLoS Negl Trop Dis 14(6):e0008323 (2020/06) [PubMed] doi: 10.1371/journal.pntd.0008323
Teklemichael AA, Mizukami S, Toume K, Mosaddeque F, Kamel MG, Kaneko O, Komatsu K, Karbwang J, Huy NT, Hirayama K. Anti-malarial activity of traditional Kampo medicine Coptis rhizome extract and its major active compounds. Malar J 19: 204 (2020/06) [PubMed] doi: 10.1186/s12936-020-03273-x
Subudhi A, O'Donnel A, Ramaprasad A, Abkallo H, Kaushik A, Ansari H, Mohamed A, Rached F, Kaneko O, Culleton R, Reece S, Pain A. Malaria parasites regulate intra-erythrocytic development duration via serpentine receptor 10 to coordinate with host rhythms. Nat Commun 11:2763 (2020/06) [PubMed] doi: 10.1038/s41467-020-16593-y
Sulistyowaty MI, Uyen NH, Suganuma K, Chitama BYA, Yahata K, Kaneko O, Sugimoto S, Yamano Y, Kawakami S, Otsuka H, Matsunami K. Six new phenylpropanoid derivatives from chemically converted extract of Alpinia galanga (L.) and anti-parasitic activities. Molecules 26(6):1756 (2021/03) [PubMed] doi: 10.3390/molecules26061756
Miyazaki S, Chitama BA, Kagaya W, Lucky AB, Zhu X, Yahata K, Morita M, Takashima E, Tsuboi T, Kaneko O. Plasmodium falciparum SURFIN4.1 forms an intermediate complex with PTEX components and Pf113 during export to the red blood cell. Parasitol Int 83:102358. (2021/04) [PubMed] doi: 10.1016/j.parint.2021.102358
Nugraheni YR, Arnuphapprasert A, Nguyen TT, Narapakdeesakul D, Nguyen HLA, Poofery J, Kaneko O, Asada M, Kaewthamasorn M. Myzorhynchus series of Anopheles mosquitoes as potential vectors of Plasmodium bubalis in Thailand. Sci Rep 5747. (2022/04) [PubMed] doi: 10.1038/s41598-022-09686-9
Christensen P, Racklyeft A, Ward KE, Matheson J, Suwanarusk R, Chua ACY, Kaneko O, Aung HL, Rénia L, Amanzougaghene, Magneron V, Lemaitre J, Le Grand R, Kyle D, Bifani P, Cook GM, Snounou G, Russell B. Improving in vitro continuous cultivation of Plasmodium cynomolgi, a model for P. vivax. Parasitol Int 102589. (2022/04) [PubMed] doi: 10.1016/j.parint.2022.102589
Poofery J, Ngamprasertwong T, Narapakdeesakul D, Arnuphapprasert A, Nugraheni YR, Thanee S, Asada M, Kaneko O, Kaewthamasorn M. Complete mitochondrial genome analyses confirm that bat Polychromophilus and ungulate Plasmodium constitute a distinct clade independent of other Plasmodium species. Sci Rep 13: 20258. (2023/11) [PubMed]
[Others articles unrelated to Plasmodium]
Hakimi H, Templeton TJ, Sakaguchi M, Yamagishi J, Miyazaki S, Yahata K, Uchihashi T, Kawazu S-I, Kaneko O, Asada M. Novel Babesia bovis exported proteins that modify properties of infected red blood cells. PLoS Pathog 16(10): e1008917 (2020/10) [PubMed] doi: 10.1371/journal.ppat.1008917
Hakimi H, Ishizaki T, Kegawa Y, Kaneko O, Kawazu S, Asada M. Genome editing of Babesia bovis using the CRISPR/Cas9 system. mSphere 4(3):e00109-19 (2019/06) [PubMed]
Hakimi H, Yamagishi J, Kegawa Y, Kaneko O, Kawazu SI, Asada M. Establishment of transient and stable transfection systems for Babesia ovata. Parasit Vectors 9:171 (2016/03)
Asada M, Yahata K, Hakimi H, Yokoyama N, Igarashi I, Kaneko O, Suarez CE, Kawazu SI. Transfection of Babesia bovis by double selection with WR99210 and Blasticidin-S and its application for functional analysis of thioredoxin peroxidase-1. PLoS One 10(5):e0125993 (2015/05) [PubMed]
Mossaad E, Asada M, Nakatani D, Inoue N, Yokoyama N, Kaneko O, Kawazu SI. Calcium ions are involved in egress of Babesia bovis merozoites from bovine erythrocytes. J Vet Med Sci. 2014 Oct 7. [Epub ahead of print] [PubMed]
Pandey K, Pandey BD, Mallik AK, Acharya J, Kato K, Kaneko O, Ferreira PE. A new molecular surveillance system for leishmaniasis. Am J Trop Med Hyg 90(6):1082-6 (2014/06) [PubMed]
Pandey BD, Pun SB, Kaneko O, Pandey K, Hirayama K. Expansion of Visceral Leishmaniasis to Western Hilly Part of Nepal. Am J Trop Med Hyg 84(1):107-8 (2011/01). [PubMed]
Pandey K, Pandey BD, Mallik AK, Kaneko O, Uemura H, Kanbara H, Yanagi T, Hirayama K. Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa’s solution-stained slides. Parasitol Res 2010 Aug;107(3):727-30. [PubMed]
Pandey BD, Pandey K, Kaneko O, Yanagi T, Hirayama K. Relapse of Visceral Leishmaniasis after Miltefosine Treatment in a Nepalese Patient. Am J Trop Med Hyg 80(4):580-2 (2009/04) [PubMed]
Pandey K, Pant S, Kanbara H, Shuaibu MN, MallikAK, Pandey BD, Kaneko O, Yanagi T. Molecular detection of Leishmania parasites from whole bodies of sandflies collected in Nepal.
Parasitol Res 103(2):293-7 (2008). [PubMed]
Katakura K, Fujise H, Takeda K, Kaneko O, Torii M, Suzuki M, Chang KP,
Hashiguchi Y. Overexpression of LaMDR2, a novel multidrug resistance
ATP-binding cassette transporter, causes 5-fluorouracil resistance in Leishmania amazonensis. FEBS Let
561(1-3):207-12 (2004). [PubMed]
Review Article
Tsuboi T, Tachibana M, Kaneko O, Torii M. Transmission-blocking vaccine of vivax malaria.Parasitol Int 52(1):1-11
(2003) [PubMed] |
